Molecular detection of Toxoplasma gondii from aborted fetuses of sheep, goats and cattle in Bangladesh

被引：16
|
作者：
Sah, Ramesh Prasad [1 ,3 ]
Dey, Anita Rani [1 ]
Rahman, A. K. M. Anisur [2 ]
Alam, Mohammad Zahangir [1 ]
Talukder, Md Hasanuzzaman [1 ]
机构：
[1] Bangladesh Agr Univ, Dept Parasitol, Mymensingh 2202, Bangladesh
[2] Bangladesh Agr Univ, Dept Med, Mymensingh 2202, Bangladesh
[3] Nepal Agr Res Council, Agr Res Stn, Kathmandu, Nepal
来源：
VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS | 2019年 / 18卷
关键词：
Aborted fetus; Toxoplasma gondii; Sheep; Goat; Cattle; PCR; Bangladesh; POLYMERASE-CHAIN-REACTION; VERTICAL TRANSMISSION; NEOSPORA-CANINUM; PCR; DIAGNOSIS; DNA; SEROPREVALENCE; INFECTION; ANIMALS; OVINE;
D O I：
10.1016/j.vprsr.2019.100347
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The study was planned to apply the PCR method for detection of T. gondii infection in sheep, goats and cattle aborted fetuses from Mymensingh, Bangladesh. A total of 58 fetal tissue samples (brain, liver, heart, skeletal muscle and placenta) of sheep (5), goats (5) and cattle (2) were selected for study. Aborted fetuses were taken from serologically positive mothers by indirect ELISA. Among them 24 and 34 samples were subjected for PCR assay by using TgB1 and TgTox4 primers respectively. DNA fragments were visualized under UV illumination after gel run. The results demonstrated 15.52% tissue samples from sheep and goat aborted fetuses were positive for T. gondii parasite. Among different tissue samples, brain, liver and heart showed presence of T. gondii parasite. None of tissue samples showed positive in case of cattle. The results of the PCR exhibited that T. gondii infection might be considered as one of the major causative agents for abortion in ewes and does. Further studies are needed to improve our knowledge on different genotypes of T. gondii that infect sheep, goat and cattle population in Bangladesh.
引用
收藏
页数：5
相关论文
共 50 条
  • [1] Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh
    Hasan, Tanjila
    Mannan, Abdul
    Hossain, Delower
    Rekha, Azizunnesa
    Hossan, Md. Monir
    Alim, Mohammad Abdul
    Uddin, A. H. M. Musleh
    [J]. VETERINARY WORLD, 2021, 14 (09) : 2386 - 2391
  • [2] Molecular detection of Toxoplasma gondii infection in slaughtered ruminants (sheep, goats and cattle) in Northwest Tunisia
    Amdouni, Yosra
    Rjeibi, Mohamed Ridha
    Rouatbi, Mariem
    Amairia, Safa
    Awadi, Sofia
    Gharbi, Mohamed
    [J]. MEAT SCIENCE, 2017, 133 : 180 - 184
  • [3] Molecular detection of Toxoplasma gondii in the slaughter sheep and goats from North India
    Kalambhe, Deepali
    Gill, J. P. S.
    Singh, Balbir Bagicha
    [J]. VETERINARY PARASITOLOGY, 2017, 241 : 35 - 38
  • [4] Seroprevalence of Toxoplasma gondii in cattle, sheep and goats in Iran
    HashemiFesharki, R
    [J]. VETERINARY PARASITOLOGY, 1996, 61 (1-2) : 1 - 3
  • [5] Age-Specificity of Toxoplasma gondii Seroprevalence in Sheep, Goats and Cattle on Subsistence Farms in Bangladesh
    Rahman, Moizur
    Azad, Md. Thoufic Anam
    Nahar, Lovely
    Rouf, Shah Md. Abdur
    Ohya, Kenji
    Chiou, Shih-Pin
    Baba, Minami
    Kitoh, Katsuya
    Takashima, Yasuhiro
    [J]. JOURNAL OF VETERINARY MEDICAL SCIENCE, 2014, 76 (09): : 1257 - 1259
  • [6] Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran
    Habibi, G. R.
    Imani, A. R.
    Gholami, M. R.
    Hablolvarid, M. H.
    Behroozikhah, A. M.
    Lotfi, M.
    Kamalzade, M.
    Najjar, E.
    Esmaeil-Nia, K.
    Bozorgi, S.
    [J]. IRANIAN JOURNAL OF PARASITOLOGY, 2012, 7 (03) : 64 - 72
  • [7] TOXOPLASMA GONDII IN RUMINANT SPECIES (CATTLE, SHEEP, AND GOATS) FROM SOUTHERN SPAIN
    Garcia-Bocanegra, I.
    Cabezon, O.
    Hernandez, E.
    Martinez-Cruz, M. S.
    Martinez-Moreno, A.
    Martinez-Moreno, J.
    [J]. JOURNAL OF PARASITOLOGY, 2013, 99 (03) : 438 - 440
  • [8] Toxoplasma gondii in Aborted Fetuses of Sheep in Ardebil Area, North-West of Iran
    Shahbazi, Gholamreza
    Hoghooghi Rad, Nasser
    Madani, Rassoul
    Matin, Somaieh
    Mortazavi, Pedjman
    Jangjou, Amir Hossein
    [J]. IRANIAN JOURNAL OF PARASITOLOGY, 2019, 14 (03) : 430 - 435
  • [9] Molecular Genotyping of Toxoplasma gondii in Sheep Aborted Fetuses Reveals Predominance of Type I Infection in Southwest of Iran
    Arefkhah, Nasir
    Sarkari, Bahador
    Asgari, Qasem
    Moshfe, Abdolali
    Khalafi, Mohammad Hasan
    Mohammadpour, Iraj
    [J]. IRANIAN JOURNAL OF PARASITOLOGY, 2020, 15 (03) : 374 - 382
  • [10] Serological and molecular detection of Toxoplasma gondii in sheep and goats in Kashan, Central Iran
    Rasti, Sima
    Marandi, Nader
    Abdoli, Amir
    Delavari, Mahdi
    Mousavi, Seyed Gholam Abbas
    [J]. JOURNAL OF FOOD SAFETY, 2018, 38 (02)
12345