The occurrence of deoxynivaloenol (DON), T-2 toxin and fumonisin B-1 was investigated in different wheat flour types, graham flour and corn flour. A total of 36 samples of different flours were collected from 2002 to 2004 from the retail market in Croatia. The contents of DON and fumonisin 131 were determined by high performance liquid cromatography and that of T-2 toxin by gas chromatography/mass spectrometry. T-2 toxin and fumonisin B-1 were not detected in any sample. DON was detected with an incidence rate of 77.78% over all samples for all years. The contamination level varied from year to year and from sample to sample and was in the range from 0 to 0.53 mg kg(-1). The EC is discussing the setting of limits for DON, such as 0.5 mg kg(-1) for cereal products for direct consumption, and 0.75 mg kg(-1) for raw cereals. Croatia has set a limit for DON of 2 mg kg(-1) for cereals and rice, and 4 mg kg(-1) for combination of fumonisins B-1, B-2 and B-3 for corn, corn flour, corn products and rice. Croatia is still lacking legislation for T-2 toxin for human consumption.
