In vivo vascular engineering of vein grafts: Directed migration of smooth muscle cells by perivascular release of elastase limits neointimal proliferation

被引:21
|
作者
Amabile, PG
Wong, H
Uy, M
Boroumand, S
Elkins, CJ
Yuksel, E
Waugh, JM
Dake, MD
机构
[1] Stanford Univ, Dept Cardiovasc & Intervent Radiol, Stanford, CA 94305 USA
[2] Hop St Marguerite, Dept Vasc Surg, Marseille, France
[3] Baylor Coll Med, Dept Surg, Houston, TX 77030 USA
关键词
atherosclerosis; blood vessels; flow dynamics; grafts; stenosis or thrombosis; intimal hyperplasia; smooth muscle cells;
D O I
10.1016/S1051-0443(07)61848-X
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
PURPOSE: Saphenous vein bypass grafting for coronary revascularization procedures remains limited by accelerated neointima formation. It was hypothesized that creation of a modified chemotactic gradient in vivo could guide migration of smooth muscle cells (SMCs) peripherally instead of in a luminal direction and reduce intimal hyperplasia during vein graft arterialization. MATERIALS AND METHODS: Surgical bypass vein grafting to femoral arteries was performed in adult male New Zealand White rabbits (n = 8 per treatment group; five for 7 d and three for 28 d). Controlled-release microspheres delivering elastase or buffered polymer only were administered perivascularly at the vein graft site. At 7 days, five vein grafts per group were harvested and cross-sections were immunostained with anti-proliferating cell nuclear antigen (PCNA) to determine the number and distribution of proliferating SMCs. At 28 days, three vein grafts per group were harvested and intima-to-media (I/M) ratios were calculated after staining with Verhoeff von Gieson-Masson trichrome stain. RESULTS: Significant early outward-directed elastin degradation resulted from elastase treatment. Concurrently, proliferating SMCs migrated peripherally. PCNA(+) cells in the outer half of the wall increased 2.37 fold compared to procedural controls (P < .0001). Directional shifts in SMC migration underlie these results because overall SMC proliferation was not significantly different. At 28 days after vein graft surgery, a 38% reduction (P = .0008) in neointima was observed relative to procedural controls. CONCLUSION: Directional guidance of SMC responses through perivascular elastase release achieves favorable vein graft remodeling characteristics, including limited neointima development. This represents practical evidence that SMC migration can be directionally guided in vivo in a vein graft model and that plaque progression can be prevented by redistributing elastin without decreasing functional vein graft wall stability.
引用
收藏
页码:709 / 715
页数:7
相关论文
共 50 条
  • [1] Perivascular controlled release of elastase limits neointimal formation in the angioplastied artery by directing migration of proliferating smooth muscle cells away from lumen
    Wong, AH
    Amabile, PG
    Dake, MD
    Waugh, JM
    [J]. CIRCULATION, 2000, 102 (18) : 246 - 246
  • [2] In vivo vascular engineering:: Directed migration of smooth muscle cells to limit neointima
    Wong, AH
    Waugh, JM
    Amabile, PG
    Yuksel, E
    Dake, MD
    [J]. TISSUE ENGINEERING, 2002, 8 (02): : 189 - 199
  • [3] Directed migration of smooth muscle cells to engineer plaque-resistant vein grafts
    Amabile, PG
    Wang, DS
    Kao, EY
    Lee, J
    Elkins, CJ
    Yuksel, E
    Hilfiker, PR
    Waugh, JM
    Dake, MD
    [J]. JOURNAL OF ENDOVASCULAR THERAPY, 2005, 12 (06) : 667 - 675
  • [4] Cantharidin inhibits neointimal hyperplasia by suppressing inflammation and the proliferation and migration of vascular smooth muscle cells
    Qiu, Liqiang
    Xia, Hao
    Jiang, Hong
    Xu, Changwu
    [J]. JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2018, 72 (16) : C33 - C33
  • [5] MicroRNA-29b Inhibits Migration and Proliferation of Vascular Smooth Muscle Cells in Neointimal Formation
    Lee, Jiyun
    Lim, Soyeon
    Song, Byeong-Wook
    Cha, Min-Ji
    Ham, Onju
    Lee, Se-Yeon
    Lee, Changyoun
    Park, Jun-Hee
    Bae, Yoonjin
    Seo, Hyang-Hee
    Seung, Minji
    Choi, Eunhyun
    Hwang, Ki-Chul
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 2015, 116 (04) : 598 - 608
  • [6] MIGRATION AND PROLIFERATION OF VASCULAR SMOOTH-MUSCLE CELLS
    BETZ, E
    [J]. ARZNEIMITTEL-FORSCHUNG/DRUG RESEARCH, 1990, 40-1 (3A): : 362 - 365
  • [7] Viscolin Inhibits In Vitro Smooth Muscle Cell Proliferation and Migration and Neointimal Hyperplasia In Vivo
    Chen, Chin-Chuan
    Liang, Chan-Jung
    Leu, Yann-Lii
    Chen, Yuh-Lien
    Wang, Shu-Huei
    [J]. PLOS ONE, 2016, 11 (12):
  • [8] Inhibition of neointimal smooth muscle cell proliferation in vein grafts by polymeric periadventitial slow delivery of verapamil
    Brauner, R
    Laks, H
    Bhuta, S
    Shvartz, O
    Golomb, G
    [J]. CIRCULATION, 1996, 94 (08) : 1722 - 1722
  • [9] Histone Deacetylase 4 Controls Neointimal Hyperplasia via Stimulating Proliferation and Migration of Vascular Smooth Muscle Cells
    Usui, Tatsuya
    Morita, Tomoka
    Okada, Muneyoshi
    Yamawaki, Hideyuki
    [J]. HYPERTENSION, 2014, 63 (02) : 397 - +
  • [10] Insulin inhibits neointimal growth by decreasing vascular smooth muscle cell migration in vivo
    Chan, KK
    Dhaliwall, J
    Al Koudsi, N
    Lam, L
    De Souza, M
    Bendeck, MP
    Giacca, A
    [J]. DIABETES, 2004, 53 : A192 - A192