A whole-genome assembly of Drosophila

被引：1075
|
作者：
Myers, EW
Sutton, GG
Delcher, AL
Dew, IM
Fasulo, DP
Flanigan, MJ
Kravitz, SA
Mobarry, CM
Reinert, KHJ
Remington, KA
Anson, EL
Bolanos, RA
Chou, HH
Jordan, CM
Halpern, AL
Lonardi, S
Beasley, EM
Brandon, RC
Chen, L
Dunn, PJ
Lai, ZW
Liang, Y
Nusskern, DR
Zhan, M
Zhang, Q
Zheng, XQ
Rubin, GM
Adams, MD
Venter, JC
机构：
[1] Celera Genom Inc, Rockville, MD 20850 USA
[2] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley Drosophila Genome Project, Berkeley, CA 94720 USA
来源：
SCIENCE | 2000年 / 287卷 / 5461期
关键词：
D O I：
10.1126/science.287.5461.2196
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We report on the quality of a whole-genome assembly of Drosophila melanogaster and the nature of the computer algorithms that accomplished it. Three independent external data sources essentially agree with and support the assembly's sequence and ordering of contigs across the euchromatic portion of the genome. In addition, there are isolated contigs that we believe represent nonrepetitive pockets within the heterochromatin of the centromeres. Comparison with a previously sequenced 2.9-megabase region indicates that sequencing accuracy within nonrepetitive segments is greater than 99.99% without manual curation. As such, this initial reconstruction of the Drosophila sequence should be of substantial value to the scientific community.
引用
收藏
页码：2196 / 2204
页数：9
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