Determination of ethanol in alcoholic drinks using an enzyme biosensor containing alcohol dehydrogenase

被引：0
|
作者：
Polan, V. [1 ]
Vytras, K. [1 ]
机构：
[1] Univ Pardubice, Fac Chem Technol, Dept Analyt Chem, Studentska 573, Pardubice 53210, Czech Republic
来源：
MICROORGANISMS IN INDUSTRY AND ENVIRONMENT: FROM SCIENTIFIC AND INDUSTRIAL RESEARCH TO CONSUMER PRODUCTS | 2011年
关键词：
ethanol biosensor; alcohol dehydrogenase; alcoholic drinks analysis; AMPEROMETRIC BIOSENSOR; HYPOXANTHINE; BEVERAGES; ELECTRODE; GLUCOSE;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The enzyme biosensor has been developed based on screen-printed carbon electrode (SPCE) containing rhodium dioxide as a mediator and alcohol dehydrogenase as a biocatalyst. Enzyme was immobilized on the electrode surface by electropolymerization of m-phenylenediamine. Resulting biosensor have been tested in model samples of ethanol. In such applications, the biosensor showed a linear response in the range of 15-120 g L-1 ethanol with a detection limit of 3.3 gL(-1) (3 sigma) and a response time of 19 s. The sensor was applied for analyses of selected alcoholic drinks with very good results.
引用
收藏
页码：688 / 692
页数：5
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