Demineralized Dentin Matrix Induces Odontoblastic Differentiation of Dental Pulp Stem Cells

被引:32
|
作者
Liu, Guolin [1 ,3 ]
Xu, Guoquan [1 ,4 ]
Gao, Zhenhua [1 ]
Liu, Zhenhai [1 ]
Xu, Junji [1 ]
Wang, Jinsong [1 ]
Zhang, Chunmei [1 ]
Wang, Songlin [1 ,2 ]
机构
[1] Capital Med Univ, Sch Stomatol, Beijing Key Lab Tooth Regenerat & Funct Reconstru, Mol Lab Gene Therapy & Tooth Regenerat, Tian Tan Xi Li 4, Beijing 100050, Peoples R China
[2] Capital Med Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Tian Tan Xi Li 4, Beijing 100050, Peoples R China
[3] Cangzhou Cent Hosp, Cangzhou, Peoples R China
[4] North China Univ Sci & Technol, Affiliated Hosp, Tangshan, Peoples R China
关键词
Demineralized dentin matrix; Dental pulp stem cell; Biomaterial; Osteogenesis; Dentinogenesis; GROWTH-FACTORS; IN-VITRO; SCAFFOLD MATERIAL; MESSENGER-RNA; TGF-BETA; REGENERATION; TISSUE; EXPRESSION; PROPERTY; MODEL;
D O I
10.1159/000440952
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
The aim of this study was to investigate the effect of demineralized dentin matrix (DDM) on dental pulp stem cells (DPSCs) and the potential of complexes with DPSCs and DDM for mineralized tissue formation. Stem cells derived from the dental pulp of healthy pigs aged 18 months were isolated and cultured. DPSCs were incubated with alpha minimum essential medium treated with DDM extract at 1 mg/ml (DDM1) or 10 mg/ml (DDM10). The concentrations of 3 growth factors in DDM extract was measured by enzyme-linked immunosorbent assay. Adhesion of DPSCs on DDM and hydroxyapatite-tricalcium phosphate (HA-TCP) surfaces was observed using scanning electron microscopy. Cell proliferation was evaluated with cell counting kit-8 and migration by Transwell migration assays. Odontoblastic differentiation was assessed by alkaline phosphatase (ALP) and alizarin red staining, ALP activity and real-time polymerase chain reaction analysis of markers of ALP, runt-related transcription factor 2, type I collagen, dentin matrix acidic phosphoprotein-1, osteonectin and dentin sialophosphoprotein (DSPP). Finally, DPSCs were combined with DDM and placed subcutaneously in nude mice for 12 weeks; DPSCs combined with HA-TCP and DDM alone served as controls. DDM could promote DPSC adhesion, migration and odontoblastic differentiation. Mineralized tissue formation was observed with the DPSC and DDM combination and the DPSC and HATCP combination. The mineralized tissue of the DPSC + DDM combination stained positive for DSPP, similar to the dentin tissue. These results indicate that DDM induces DPSC odontoblastic differentiation, suggesting applications for dentin regeneration. (C) 2015 S. Karger AG, Basel
引用
收藏
页码:65 / 76
页数:12
相关论文
共 50 条
  • [1] Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells
    Shoko Fujino
    Sayuri Hamano
    Atsushi Tomokiyo
    Risa Sugiura
    Daiki Yamashita
    Daigaku Hasegawa
    Hideki Sugii
    Shinsuke Fujii
    Tomohiro Itoyama
    Hirofumi Miyaji
    Hidefumi Maeda
    [J]. Scientific Reports, 13
  • [2] Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells
    Fujino, Shoko
    Hamano, Sayuri
    Tomokiyo, Atsushi
    Sugiura, Risa
    Yamashita, Daiki
    Hasegawa, Daigaku
    Sugii, Hideki
    Fujii, Shinsuke
    Itoyama, Tomohiro
    Miyaji, Hirofumi
    Maeda, Hidefumi
    [J]. SCIENTIFIC REPORTS, 2023, 13 (01)
  • [3] Hyaluronan induces odontoblastic differentiation of dental pulp stem cells via CD44
    Naoki Umemura
    Emika Ohkoshi
    Masamichi Tajima
    Hirotaka Kikuchi
    Tadashi Katayama
    Hiroshi Sakagami
    [J]. Stem Cell Research & Therapy, 7
  • [4] Hyaluronan induces odontoblastic differentiation of dental pulp stem cells via CD44
    Umemura, Naoki
    Ohkoshi, Emika
    Tajima, Masamichi
    Kikuchi, Hirotaka
    Katayama, Tadashi
    Sakagami, Hiroshi
    [J]. STEM CELL RESEARCH & THERAPY, 2016, 7
  • [5] Quaking promotes the odontoblastic differentiation of human dental pulp stem cells
    Li, Shuchen
    Lin, Chujiao
    Zhang, Jie
    Tao, Huangheng
    Liu, Huan
    Yuan, Guohua
    Chen, Zhi
    [J]. JOURNAL OF CELLULAR PHYSIOLOGY, 2018, 233 (09) : 7292 - 7304
  • [6] Senescence and odontoblastic differentiation of dental pulp cells
    Nozu, Aoi
    Hamano, Sayuri
    Tomokiyo, Atsushi
    Hasegawa, Daigaku
    Sugii, Hideki
    Yoshida, Shinichiro
    Mitarai, Hiromi
    Taniguchi, Shuntaro
    Wada, Naohisa
    Maeda, Hidefumi
    [J]. JOURNAL OF CELLULAR PHYSIOLOGY, 2019, 234 (01) : 849 - 859
  • [7] Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts
    A Almushayt
    K Narayanan
    A E Zaki
    A George
    [J]. Gene Therapy, 2006, 13 : 611 - 620
  • [8] Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts
    Almushayt, A
    Narayanan, K
    Zaki, AE
    George, A
    [J]. GENE THERAPY, 2006, 13 (07) : 611 - 620
  • [9] Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells
    Yoshida, S.
    Wada, N.
    Hasegawa, D.
    Miyaji, H.
    Mitarai, H.
    Tomokiyo, A.
    Hamano, S.
    Maeda, H.
    [J]. JOURNAL OF DENTAL RESEARCH, 2016, 95 (11) : 1282 - 1290
  • [10] Treated dentin matrix induces odontogenic differentiation of dental pulp stem cells via regulation of Wnt/β-catenin signaling
    Liu, Sirui
    Sun, Jingjing
    Yuan, Shuai
    Yang, Yanyu
    Gong, Yuping
    Wang, Ying
    Guo, Runying
    Zhang, Xue
    Liu, Yiming
    Mi, Hongyan
    Wang, Meiyue
    Liu, Mengzhe
    Li, Rui
    [J]. BIOACTIVE MATERIALS, 2022, 7 : 85 - 97