Identification and characterization of the two-component NtrY/NtrX regulatory system in Azospirillum brasilense

被引：36

作者：

Ishida, ML

Assumpçao, MC

Machado, HB

Benelli, EM

Souza, EM

Pedrosa, FO

机构：

[1] UFPR, Dept Bioquim & Biol Mol, BR-81531990 Curitiba, Parana, Brazil

[2] UFPR, Dept Farmacol, BR-81531990 Curitiba, Parana, Brazil

来源：

BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH | 2002年 / 35卷 / 06期

关键词：

Azospirillum brasilense; two-component regulatory system; nitrogen regulation; ntrYX genes; ntrBC genes;

D O I：

10.1590/S0100-879X2002000600004

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Two Azospirillum brasilense open reading frames (ORFs) exhibited homology with the two-component NtrY/NtrX regulatory system from Azorhizobium caulinodans. These A. brasilense ORFs, located downstream to the nifR3ntrBC operon, were isolated, sequenced and characterized. The present study suggests that ORF1 and ORF2 correspond to the A. brasilense ntrY and ntrX genes, respectively. The amino acid sequences of A. brasilense NtrY and NtrX proteins showed high similarity to sensor/kinase and regulatory proteins, respectively. Analysis of lacZ transcriptional fusions by the beta-galactosidase assay in Escherichia coli ntrC mutants showed that the NtrY/NtrX proteins failed to activate transcription of the nifA promoter of A. brasilense. The ntrYX operon complemented a nifR3ntrBC deletion mutant of A. brasilense for nitrate-dependent growth, suggesting a possible crosstalk between the NtrY/X and NtrB/C sensor/regulator pairs. Our data support the existence of another two-component regulatory system in A. brasilense, the NtrY/NtrX system, probably involved in the regulation of nitrate assimilation.

引用

页码：651 / 661

页数：11

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