Cultures are increasingly recognised for their inherent value, yet, despite political and societal concern, culture is widely unrecognised in assessment techniques. Life cycle sustainability assessment (LCSA), a technique encompassing environmental, social and economic aspects, is growing in popularity. However, cultural values are rarely considered in LCSA. This paper reviews the meaning of culture; current efforts to include culture in environmental life cycle assessment (LCA), social LCA (S-LCA) and LCSA; and aspects to address when investigating integration of culture in LCA, S-LCA and LCSA. A literature review was undertaken on definitions of culture, recognition of culture in policy and decision making, and how culture is incorporated into assessment techniques. The potential for integrating culture in LCSA was evaluated in terms of the potential benefits and challenges. Culture is often intangible and inaccessible, which may then lead to a lack of recognition in decision-making processes, or if it is recognised, then it is relegated as an afterthought. Explicitly including consideration of culture within LCSA will allow its representation alongside other sustainability aspects. The challenges of representing culture within LCSA include recognising when 'culture' should be distinguished from 'social'; culture's dynamic nature; the data collection process; and the diversity of cultures between stakeholders and at different scales from community through to nation. The potential benefits of representing culture within LCSA include greater resonance of LCSA results with stakeholders; a more comprehensive decision support tool which appropriately accounts for values; and an assessment technique which may help protect communities and their diversity of cultures. Representing culture in LCSA is not straightforward and, to some extent, may be addressed through social indicators. However, developing LCSA to explicitly address cultural values has potential benefits. Future research should focus on opportunities for the development of (a) a culturally inclusive LCSA process and (b) additional cultural indicators and/or dimensions of existing LCSA indicators that represent cultural values.