A strategy to verify the absence of the pgm locus in Yersinia pestis strain candidates for select agent exemption

被引：9

作者：

Jenkins, Amy L. ^{[1
]}

Worsham, Patricia L. ^{[1
]}

Welkos, Susan L. ^{[1
]}

机构：

[1] USA, Med Res Inst Infect Dis, Bacteriol Div, Frederick, MD 21702 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2009年 / 77卷 / 03期

关键词：

Yersinia pestis; Pgm locus; Polymerase chain reaction; Select agent rules; IRP2; GENE; PIGMENTATION; VIRULENCE; DELETION; PLAGUE; IRON; MUTANTS;

D O I：

10.1016/j.mimet.2009.02.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Yersinia pestis is a Department of Health and Human Services select agent as defined in federal regulations. Certain attenuated strains of Y. pestis, such as the pgm(-) strain, are exempt from these regulations. Herein we describe a strategy to verify the absence of the pgm locus in Y pestis strains being considered as candidates for select agent exemption by PCR analysis of virulence-associated genes. Published by Elsevier B.V.

引用

页码：316 / 319

页数：4

共 10 条

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