Improvement of Leptospira spp. diagnosis in aborted bovine fetuses by qPCR

被引：3

作者：

Cheuquepan Valenzuela, Felipe ^{[1
]}

Recavarren, Mariana ^{[2
]}

Quintana, Silvina ^{[2
,3
,4
]}

Canton, German ^{[5
]}

Odeon, Anselmo ^{[6
]}

Marin, Maia ^{[1
]}

Morrell, Eleonora ^{[5
]}

机构：

[1] CONICET INTA, Inst Innovac Prod Agr & Desarrollo Sostenible IPA, Ruta 226 Km 73-5, RA-7620 Buenos Aires, DF, Argentina

[2] Inst Anal Fares Taie, Rivadavia 3331, RA-7600 Mar Del Plata, Buenos Aires, Argentina

[3] Consejo Nacl Invest Cient & Tecn CONICET, Rivadavia 1917,C1033AAJ, RA-1917 Buenos Aires, DF, Argentina

[4] UNMDP, Ctr Invest Abejas Sociales, Dept Biol, Fac Ciencias Exactas & Nat, Funes 3350, RA-7600 Mar Del Plata, Buenos Aires, Argentina

[5] Inst Nacl Tecnol Agr INTA, Estn Expt Agr Balcarce, Ruta 226 Km 73-5, RA-7620 Buenos Aires, DF, Argentina

[6] Univ Nacl Mar del Plata, Fac Ciencias Agr, Ruta 226 Km 73-5, RA-7620 Buenos Aires, DF, Argentina

来源：

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2020年 / 73卷

关键词：

Bovine abortion; Fetuses; Diagnosis; Leptospira spp; qPCR; REAL-TIME PCR; PATHOGENIC LEPTOSPIRA;

D O I：

10.1016/j.cimid.2020.101555

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Leptospirosis is a disease with major economic impact on livestock industry. The objective of this work was to determine the presence of Leptospira spp. DNA by qPCR in bovine fetuses with presumptive diagnosis of leptospirosis as the cause of abortion. Leptospira spp. DNA was detected by qPCR in 11 out of 34 fetuses. These specimens (10/11) had histopathological findings in hepatic and/or renal tissues compatible with leptospirosis. qPCR detection rate (32.4 %) was higher compared with direct immuno-fluorescence antibody test (DFAT) (11.8 %). The concordance coefficient between both techniques was 0.44. qPCR is a rapid and sensitive technique for the diagnosis of leptospirosis and improved the detection rate in fetal tissues compared with DFAT. Implementation of molecular techniques may increase the accurate detection of leptospirosis as a cause of bovine abortion allowing the application of rapid therapeutic and prophylactics measures in order to reduce the impact of this zoonotic disease.

引用

页数：3

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