Influence of in vitro simulated gastroduodenal digestion on the antibacterial activity, metabolic profiling and polyphenols content of green tea (Camellia sinensis)

被引：46
|
作者：
Marchese, Anna [1 ]
Coppo, Erika [1 ]
Sobolev, Anatoly P. [2 ]
Rossi, Daniela
Mannina, Luisa [2 ,4 ]
Daglia, Maria [3 ]
机构：
[1] Univ Genoa, Microbiol Unit DISC, I-16132 Genoa, Italy
[2] CNR, Ist Metodol Chim, Labo Risonanza Magnet Annalaura Segre, I-00015 Rome, Italy
[3] Univ Pavia, Dept Drug Sci, Med Chem & Pharmaceut Technol Sect, I-27100 Pavia, Italy
[4] Univ Roma La Sapienza, Dipartimento Chim & Tecnol Farm, I-00185 Rome, Italy
来源：
FOOD RESEARCH INTERNATIONAL | 2014年 / 63卷
关键词：
Green tea; Gastroduodenal digestion; Catechins; Antibacterial activity; NMR; HPLC-PAD-ESI/MS; RESISTANT STAPHYLOCOCCUS-AUREUS; HIGH-FIELD NMR; EPIGALLOCATECHIN GALLATE; H-1; INHIBITION; BACTERIA; LETTUCE; EGCG;
D O I：
10.1016/j.foodres.2014.01.036
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The antistaphylococcal activity as well as the metabolic profiling and polyphenols content of green tea (Camellia sinensis) before and after in vitro simulated gastric, duodenal and gastroduodenal digestion were investigated. Gastric and duodenal digested samples showed antistaphylococcal activity, whereas gastroduodenal digested samples did not show any antibacterial activity. Metabolite analysis, carried out using an explorative untargeted NMR-based approach and a RP-HPLC-PAD-ESI-MSn method, showed that green tea polyphenols are stable under gastric conditions. Duodenal digested sample maintained the antibacterial activity, even if some polyphenols are widely degraded. Epicatechin 3-gallate, under duodenal digestive conditions, is hydrolyzed to produce epicatechin, whereas epigallocatechin 3-gallate reacts with digestive enzymes and a galloyl-high molecular weight derivative is produced. Gastroduodenal digestion results in degradation of polyphenols, especially gallocatechins, considered the main responsible for the antibacterial activity. These results explain the loss of activity of gastroduodenal digested samples and why in vivo green tea has neither protective nor therapeutic effects against intestinal and systemic bacterial infections. (C) 2014 Published by Elsevier Ltd.
引用
收藏
页码：182 / 191
页数：10
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