Effect of silkworm hemolymph on the expression of E-coli beta-galactosidase in insect cell lines infected with recombinant baculoviruses

被引：0

作者：

Woo, SD ^{[1
]}

Kim, WJ ^{[1
]}

Kim, HS ^{[1
]}

Choi, JY ^{[1
]}

Jin, BR ^{[1
]}

Kang, SK ^{[1
]}

机构：

[1] SEOUL NATL UNIV,COLL AGR & LIFE SCI,DEPT AGR BIOL,SUWON 441744,SOUTH KOREA

来源：

MOLECULES AND CELLS | 1997年 / 7卷 / 04期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of silkworm hemolymph on the expression of foreign genes by recombinant baculoviruses in cell lines were studied, The expression efficiency of beta-galactosidase by recombinant virus containing the E. coli lacZ gene at various concentrations of hemolymph and FBS was determined in BmN and Sf cell lines, The addition of hemolymph to the medium containing FBS accelerated the expression of beta-galactosidase by recombinant viruses in both cells. It was more effective in BmN cells than in Sf cells. Hemolymph was most effective in enhancing virus multiplicity under conditions of 5% FBS.

引用

页码：572 / 574

页数：3

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