Comparative analysis of GDF 8 (myostatin) in Bos indicus and Bos taurus

被引：5
|
作者：
Tantia, Madhu S.
Vijh, Ramesh K.
Kumar, S. T. Bharani
Mishra, Bina
Reecy, James M.
机构：
[1] Natl Bur Anim Genet Resources, Karnal 132001, Haryana, India
[2] Iowa State Univ, Dept Anim Sci, Ames, IA USA
来源：
DNA SEQUENCE | 2006年 / 17卷 / 04期
关键词：
myostatin; cattle; indicus; sequence;
D O I：
10.1080/10425170600807603
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We report the myostatin gene sequence of Bos indicus cattle in comparison to Bos taurus. B. indicus genomic sequence was obtained by overlapping PCR amplification of genomic DNA. Exon splice sites were confirmed by mRNA sequencing. There were 5 exonic single nucleotide polymorphisms (SNP) only one of which was a non-synonymous mutation that resulted in a serine to asparagine (S214N) amino acid substitution. The B. indicus gene has two insertions of 16 and 12 bases in the first intron. In addition, SNPs in the 3' UTR and intronic regions are also reported.
引用
收藏
页码：311 / 313
页数：3
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