Vaccination with Recombinant Adenoviruses Expressing the Peste des Petits Ruminants Virus F or H Proteins Overcomes Viral Immunosuppression and Induces Protective Immunity against PPRV Challenge in Sheep

被引：45

作者：

Rojas, Jose M. ^{[1
]}

Moreno, Hector ^{[1
]}

Valcarcel, Felix ^{[1
]}

Pena, Lourdes ^{[1
]}

Sevilla, Noemi ^{[1
]}

Martin, Veronica ^{[1
]}

机构：

[1] Ctr Invest Sanidad Anim CISA INIA, Inst Nacl Invest & Tecnol Agr & Alimentaria, Madrid, Spain

来源：

PLOS ONE | 2014年 / 9卷 / 07期

关键词：

RINDERPEST VIRUS; RESPONSES; VECTORS; ANTIBODY; GOATS; IMMUNIZATION; INDUCTION; INFECTION; EFFICACY; VACCINES;

D O I：

10.1371/journal.pone.0101226

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Peste des petits ruminants (PPR) is a highly contagious disease of small ruminants caused by the Morbillivirus peste des petits ruminants virus (PPRV). Two recombinant replication-defective human adenoviruses serotype 5 (Ad5) expressing either the highly immunogenic fusion protein (F) or hemagglutinin protein (H) from PPRV were used to vaccinate sheep by intramuscular inoculation. Both recombinant adenovirus vaccines elicited PPRV-specific B- and T-cell responses. Thus, neutralizing antibodies were detected in sera from immunized sheep. In addition, we detected a significant antigen specific T-cell response in vaccinated sheep against two different PPRV strains, indicating that the vaccine induced heterologous T cell responses. Importantly, no clinical signs and undetectable virus shedding were observed after virulent PPRV challenge in vaccinated sheep. These vaccines also overcame the T cell immunosuppression induced by PPRV in control animals. The results indicate that these adenovirus constructs could be a promising alternative to current vaccine strategies for the development of PPRV DIVA vaccines.

引用

页数：16