N-Acetylglucosaminyltransferase V Regulates Extravillous Trophoblast Invasion through Glycosylation of α5β1 Integrin
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Yamamoto, Eiko
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Nagoya Univ, Grad Sch Med, Dept Obstet & Gynecol, Showa Ku, Nagoya, Aichi 4668550, JapanNagoya Univ, Grad Sch Med, Dept Obstet & Gynecol, Showa Ku, Nagoya, Aichi 4668550, Japan
Yamamoto, Eiko
[1
]
Ino, Kazuhiko
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机构:Nagoya Univ, Grad Sch Med, Dept Obstet & Gynecol, Showa Ku, Nagoya, Aichi 4668550, Japan
Ino, Kazuhiko
Miyoshi, Eiji
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Osaka Univ, Grad Sch Med, Dept Mol Biochem & Clin Invest, Suita, Osaka 5650871, Japan
Osaka Univ, Grad Sch Med, Dept Biochem, Suita, Osaka 5650871, JapanNagoya Univ, Grad Sch Med, Dept Obstet & Gynecol, Showa Ku, Nagoya, Aichi 4668550, Japan
Miyoshi, Eiji
[3
,4
]
Inamori, Kei-ichiro
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Osaka Univ, Grad Sch Med, Dept Biochem, Suita, Osaka 5650871, JapanNagoya Univ, Grad Sch Med, Dept Obstet & Gynecol, Showa Ku, Nagoya, Aichi 4668550, Japan
Inamori, Kei-ichiro
[4
]
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Abe, Akihiro
[2
]
Sumigama, Seiji
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机构:Nagoya Univ, Grad Sch Med, Dept Obstet & Gynecol, Showa Ku, Nagoya, Aichi 4668550, Japan
Sumigama, Seiji
Iwase, Akira
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机构:Nagoya Univ, Grad Sch Med, Dept Obstet & Gynecol, Showa Ku, Nagoya, Aichi 4668550, Japan
Iwase, Akira
Kajiyama, Hiroaki
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机构:Nagoya Univ, Grad Sch Med, Dept Obstet & Gynecol, Showa Ku, Nagoya, Aichi 4668550, Japan
Kajiyama, Hiroaki
Shibata, Kiyosumi
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机构:Nagoya Univ, Grad Sch Med, Dept Obstet & Gynecol, Showa Ku, Nagoya, Aichi 4668550, Japan
Shibata, Kiyosumi
Nawa, Akihiro
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机构:Nagoya Univ, Grad Sch Med, Dept Obstet & Gynecol, Showa Ku, Nagoya, Aichi 4668550, Japan
Nawa, Akihiro
Kikkawa, Fumitaka
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机构:Nagoya Univ, Grad Sch Med, Dept Obstet & Gynecol, Showa Ku, Nagoya, Aichi 4668550, Japan
Kikkawa, Fumitaka
机构:
[1] Nagoya Univ, Grad Sch Med, Dept Obstet & Gynecol, Showa Ku, Nagoya, Aichi 4668550, Japan
[2] Nagoya Univ, Grad Sch Med, Dept Hematol, Nagoya, Aichi 4668550, Japan
[3] Osaka Univ, Grad Sch Med, Dept Mol Biochem & Clin Invest, Suita, Osaka 5650871, Japan
[4] Osaka Univ, Grad Sch Med, Dept Biochem, Suita, Osaka 5650871, Japan
For successful human placentation, invasion of trophoblast cells into the uterus and its associated vasculature is essential, and the regulation of this process is controlled by many factors at the fetal-maternal interface. N-acetylglucosaminyltransferase V (GnT-V) is a key enzyme that catalyzes beta 1, 6-N-acetylglucosamine (beta 1-6GlcNAc) branching on asparagine-linked oligosaccharides of cell proteins. GnT-V and its product, beta 1-6GlcNAc, are known to regulate cellular transformation and correlate with the metastatic potential of various cancer cells. The aim of the present study was to determine whether extravillous trophoblast (EVT) expressed this molecule and examine the role of GnT-Vin the regulation of human trophoblast invasion. Immunohistochemistry showed that GnT-V was strongly expressed within the cytoplasm of EVT in the anchoring villi; this expression was down-regulated in EVTs invading the decidua. Suppression of beta 1-6GlcNAc glycosylation by swainsonine enhanced the migratory potential and invasive capability of both primary EVTs and the EVT cell line, HTR-8/SVneo. Down-regulation of GnT-V expression by small interfering RNA in the choriocarcinoma cell line Jar consistently enhanced the migration and invasive capacity of these cells and elevated cellular adhesion to extracellular matrix proteins, such as fibronectin and collagen type I/IV. The extent of beta 1-6 branching of alpha 5 beta 1 integrin was significantly reduced in small interfering GnT-V-transfected Jar cells compared with mock transfectants, although the expression of alpha 1, alpha 5, alpha 6, and beta 1 integrin on the cell surface was not changed. These results suggest that GnT-V is expressed in human EVT and is involved in regulating trophoblast invasion through modifications of the oligosaccharide chains of alpha 5 beta 1 integrin. (Endocrinology 150: 990-999, 2009)
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Fourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Dept Cell Biol, State Key Lab Canc Biol, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R ChinaFourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Cui, Jian
Huang, Wan
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Fourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Dept Cell Biol, State Key Lab Canc Biol, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R ChinaFourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Huang, Wan
Wu, Bo
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Collaborat Innovat Ctr Canc Med, Beijing Inst Radiat Med, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing, Peoples R ChinaFourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Wu, Bo
Jin, Jin
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Fourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Dept Cell Biol, State Key Lab Canc Biol, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R ChinaFourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Jin, Jin
Jing, Lin
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Fourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Dept Cell Biol, State Key Lab Canc Biol, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R ChinaFourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Jing, Lin
Shi, Wen-Pu
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Fourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Dept Cell Biol, State Key Lab Canc Biol, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R ChinaFourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Shi, Wen-Pu
Liu, Zhen-Yu
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Fourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Dept Cell Biol, State Key Lab Canc Biol, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R ChinaFourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Liu, Zhen-Yu
Yuan, Lin
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Fourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Dept Cell Biol, State Key Lab Canc Biol, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R ChinaFourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Yuan, Lin
Luo, Dan
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Fourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Dept Cell Biol, State Key Lab Canc Biol, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R ChinaFourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Luo, Dan
Li, Ling
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Fourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Dept Cell Biol, State Key Lab Canc Biol, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R ChinaFourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Li, Ling
Chen, Zhi-Nan
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Fourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Dept Cell Biol, State Key Lab Canc Biol, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R ChinaFourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Chen, Zhi-Nan
Jiang, Jian-Li
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Fourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China
Fourth Mil Med Univ, Dept Cell Biol, State Key Lab Canc Biol, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R ChinaFourth Mil Med Univ, Cell Engn Res Ctr, 169 Chang Le West Rd, Xian 710032, Shaanxi, Peoples R China