Antimicrobial susceptibility testing of rapidly growing mycobacteria isolated in Japan

被引:49
|
作者
Hatakeyama, Shuji [1 ,2 ,3 ]
Ohama, Yuki [4 ]
Okazaki, Mitsuhiro [5 ]
Nukui, Yoko [4 ,6 ]
Moriya, Kyoji [4 ]
机构
[1] Jichi Med Univ Hosp, Div Gen Internal Med, Shimotsuke, Tochigi 3290498, Japan
[2] Jichi Med Univ Hosp, Div Infect Dis, Shimotsuke shi, Shimotsuke, Tochigi 3290498, Japan
[3] Tokyo Univ Hosp, Dept Infect Dis, Bunkyo Ku, Tokyo 1138655, Japan
[4] Tokyo Univ Hosp, Dept Infect Control & Prevent, Bunkyo Ku, Tokyo 1138655, Japan
[5] Tokyo Univ Technol, Sch Hlth Sci, Dept Med Technol, Ota Ku, Tokyo 1448535, Japan
[6] Tokyo Med & Dent Univ, Dept Infect Control & Prevent, Bunkyo Ku, Tokyo 1138510, Japan
来源
BMC INFECTIOUS DISEASES | 2017年 / 17卷
关键词
Rapidly growing mycobacteria; Mycobacterium abscessus complex; Antimicrobial susceptibility; Tigecycline; Tebipenem; Macrolides; INTRINSIC MACROLIDE RESISTANCE; ABSCESSUS; TIGECYCLINE; INFECTION; MASSILIENSE;
D O I
10.1186/s12879-017-2298-8
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Background: Difficult-to-treat infections caused by rapidly growing mycobacteria (RGM) are increasingly observed in clinical settings. However, studies on antimicrobial susceptibilities and effective treatments against RGM in Japan are limited. Methods: We conducted susceptibility testing of potential antimicrobial agents, including tigecycline and tebipenem, against RGM. Clinical RGM isolates were collected from a university hospital in Japan between December 2010 and August 2013. They were identified using matrix-assisted laser desorption/ionization time-offlight mass spectrometry and the sequencing of 16S rRNA, rpoB, and hsp65 genes. The samples were utilized for susceptibility testing using 16 antimicrobials, with frozen broth microdilution panels. Results: Forty-two isolates were obtained: 13, Mycobacterium abscessus complex; 12, Mycobacterium chelonae; 9, Mycobacterium fortuitum; and 8, M. fortuitum group species other than M. fortuitum. Different antimicrobial susceptibility patterns were observed between RGM species. Clarithromycin-susceptible strain rates were determined to be 0, 62, and 100% for M. fortuitum, M. abscessus complex, and M. chelonae, respectively. M. abscessus complex (100%) and > 80% M. chelonae isolates were non-susceptible, while 100% M. fortuitum group isolates were susceptible to moxifloxacin. Linezolid showed good activity against 77% M. abscessus complex, 89% M. fortuitum, and 100% M. chelonae isolates. Regardless of species, all tested isolates were inhibited by tigecycline at very low minimal inhibitory concentrations (MICs) of = 0.5 mu g/mL. MICs of tebipenem, an oral carbapenem, were <= 4 mu g/mL against all M. fortuitum group isolates. Conclusions: Our study demonstrates the importance of correct identification and antimicrobial susceptibility testing, including the testing of potential new agents, in the management of RGM infections.
引用
收藏
页数:7
相关论文
共 50 条
  • [1] Antimicrobial susceptibility testing of rapidly growing mycobacteria isolated in Japan
    Shuji Hatakeyama
    Yuki Ohama
    Mitsuhiro Okazaki
    Yoko Nukui
    Kyoji Moriya
    [J]. BMC Infectious Diseases, 17
  • [2] Susceptibility to antimicrobial agents of rapidly growing mycobacteria
    Ruiz-Aragon, J.
    Garcia-Agudo, L.
    Flores, S.
    Rodriguez, M. J.
    Marin, R.
    Garcia-Martos, P.
    [J]. REVISTA ESPANOLA DE QUIMIOTERAPIA, 2007, 20 (04) : 429 - 432
  • [3] RAPIDLY GROWING MYCOBACTERIA - TESTING OF SUSCEPTIBILITY TO 34 ANTIMICROBIAL AGENTS BY BROTH MICRODILUTION
    SWENSON, JM
    THORNSBERRY, C
    SILCOX, VA
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1982, 22 (02) : 186 - 192
  • [4] Nationwide surveillance of antimicrobial susceptibility of 509 rapidly growing mycobacteria strains isolated from clinical specimens in Japan
    Keisuke Kamada
    Atsushi Yoshida
    Shigekazu Iguchi
    Yuko Arai
    Yutaka Uzawa
    Satoshi Konno
    Masahiro Shimojima
    Ken Kikuchi
    [J]. Scientific Reports, 11
  • [5] Nationwide surveillance of antimicrobial susceptibility of 509 rapidly growing mycobacteria strains isolated from clinical specimens in Japan
    Kamada, Keisuke
    Yoshida, Atsushi
    Iguchi, Shigekazu
    Arai, Yuko
    Uzawa, Yutaka
    Konno, Satoshi
    Shimojima, Masahiro
    Kikuchi, Ken
    [J]. SCIENTIFIC REPORTS, 2021, 11 (01)
  • [6] Antimicrobial susceptibility testing of rapidly growing mycobacteria by microdilution - Experience of a tertiary care centre
    Set, R.
    Rokade, S.
    Agrawal, S.
    Shastri, J.
    [J]. INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2010, 28 (01) : 48 - 50
  • [7] EVALUATION OF ETEST FOR SUSCEPTIBILITY TESTING OF RAPIDLY GROWING MYCOBACTERIA
    BIEHLE, JR
    CAVALIERI, SJ
    SAUBOLLE, MA
    GETSINGER, LJ
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (07) : 1760 - 1764
  • [8] Drug Susceptibility Testing of 31 Antimicrobial Agents on Rapidly Growing Mycobacteria Isolates from China
    Pang, Hui
    Li, Guilian
    Zhao, Xiuqin
    Liu, Haican
    Wan, Kanglin
    Yu, Ping
    [J]. BIOMED RESEARCH INTERNATIONAL, 2015, 2015
  • [9] Antimicrobial susceptibility of rapidly growing mycobacteria using the rapid colorimetric method
    I. B. Ramis
    M. Cnockaert
    A. von Groll
    C. L. Nogueira
    S. C. Leão
    E. Andre
    A. Simon
    J. C. Palomino
    P. E. A. da Silva
    P. Vandamme
    A. Martin
    [J]. European Journal of Clinical Microbiology & Infectious Diseases, 2015, 34 : 1403 - 1413
  • [10] Antimicrobial susceptibility of rapidly growing mycobacteria using the rapid colorimetric method
    Ramis, I. B.
    Cnockaert, M.
    von Groll, A.
    Nogueira, C. L.
    Leao, S. C.
    Andre, E.
    Simon, A.
    Palomino, J. C.
    da Silva, P. E. A.
    Vandamme, P.
    Martin, A.
    [J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2015, 34 (07) : 1403 - 1413