Molecular screening for Rickettsia, Anaplasmataceae and Coxiella burnetii in Rhipicephalus sanguineus ticks from Malaysia

被引：0

作者：

Watanabe, M. ^{[1
,3
]}

Nakao, R. ^{[2
]}

Amin-Babjee, S. M. ^{[1
]}

Maizatul, A. M. ^{[1
]}

Youn, J. H. ^{[2
]}

Qiu, Y. ^{[2
]}

Sugimoto, C. ^{[2
]}

Watanabe, M. ^{[1
,3
]}

机构：

[1] Univ Putra Malaysia, Dept Vet Pathol & Microbiol, Fac Vet Med, Serdang 43400, Selangor, Malaysia

[2] Hokkaido Univ, Res Ctr Zoonosis Control, Div Educ & Collaborat, Sapporo, Hokkaido 060, Japan

[3] Univ Putra Malaysia, Fac Vet Med, Dept Compan Anim Med & Surg, Serdang 43400, Selangor, Malaysia

来源：

TROPICAL BIOMEDICINE | 2015年 / 32卷 / 02期

关键词：

EHRLICHIA-CANIS; Q-FEVER; PCR; DIFFERENTIATION; SEQUENCE; STRAINS; HUMANS; BORNE; COM1; DOGS;

D O I：

暂无

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A total of 44 Rhipicephalus sanguineus ticks collected from 23 dogs from Malaysia were screened for Rickettsia, Anaplasmataceae and Coxiella burnetii. Coxiella burnetii was detected in 59% (26/44) of ticks however Rickettsia and Anaplasmataceae were not detected in any of the ticks. In order to genotype the strains of C. burnetii, multispacer sequence typing (MST) was carried out using three different spacers. One of the spacers; Cox2 successfully amplified a fragment for which the full length sequence of 397 bp was obtained. The sequenced product revealed only a single nucleotide difference with the Cox2.3 type sequence.

引用

页码：390 / 398

页数：9

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