A novel insight to screen the optimal spray-drying protectants and parameters for manufacturing lactic acid bacteria preparations

被引:9
|
作者
Zhang, Zhuo [1 ]
Peng, Sen [1 ]
Sun, Xiaoqi [1 ]
Jie, Yu [1 ]
Zhao, Hongfei [1 ,2 ]
Zhu, Baoqing [1 ,2 ]
Dziugan, Piotr [3 ]
Zhang, Bolin [1 ,2 ]
机构
[1] Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China
[2] Beijing Forestry Univ, Beijing Key Lab Forestry Food Proc & Safety, Beijing, Peoples R China
[3] Tech Univ Lodz, Inst Fermentat Technol & Microbiol, Lodz, Poland
基金
中国国家自然科学基金;
关键词
Spray drying; lactic acid bacteria; protectants; beta-galactosidase; liposome; DRIED LACTOBACILLUS-BULGARICUS; VIABILITY; SURVIVAL; POWDER; OPTIMIZATION; PRESERVATION; TREHALOSE; STORAGE; CULTURES;
D O I
10.1080/07373937.2019.1674323
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
This study constructed a beta-galactosidase (beta-gal) liposome model instead of bacterial cells to optimize spray-drying protectants and parameters. The screened protectant combination consisted of 35 g/L monosodium glutamate, 50 g/L sucrose, and 35 g/L maltodextrin. The optimal parameters for spray drying were 130 degrees C inlet air temperature, 7.5 mL/min feed flow rate, and 150 mL optimal protectant combination. The viability of the five lactic acid bacteria strains spray-dried with the optimized protectant and parameters was over 50%.Lactobacillus helveticusstrain LH-9's mechanism of resistance to spray-dry treatment was investigated in the presence of the optimal protectant combination.
引用
收藏
页码:1843 / 1856
页数:14
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