Huakuichenia soli gen. nov., sp nov., a new member of the family Microbacteriaceae, isolated from contaminated soil

A novel Gram-stain-positive, non-motile, rod-shaped bacterial strain designated LIP-1(T) was isolated from the contaminated soil of a pesticide factory in Xinyi, China, was investigated for its taxonomic allocation by a polyphasic approach. Cell growth occurred at 16-37 degrees C (optimum, 30 degrees C), in the presence of 0-2.0% (w/v) NaCI (optimum, 0%) and at pH 6.0-9.0 (optimum, pH 7.0). The major fatty acids of strain LIP-1(T) were anteiso-C-15:0 (50.8%), iSO-C-16:0, (17.6%) and anteiso-C-17:0 (17.4%). The cell-wall peptidoglycan type was B2 delta with 2,4-diaminobutyric acid as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol and two unidentified glycolipids. The major menaquinones were MK-12 and MK-11. The genomic DNA G+C content was approximately 63.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain LIP-1(T) formed a distinct clade within the radiation of the family Microbacteriaceae and had the highest sequence similarity with Microbacterium ginsengisoli Gsoil 259(T) (96.01%) followed by Cryobacterium arcticum SK1(T) (94.94%). On the basis of the phylogenetic analyses and distinct phenotypic characteristics, a new genus, namely Huakuichenia gen. nov., is proposed, harbouring the novel species Huakuichenia soli gen. nov., sp. nov. with the type strain LIP-1(T) (=CCTCC AB 2015422(T)=KCTC 39698(T)).