Fold 2D Woven DNA Origami to Origami+ Structures

DNA origami strategy has greatly promoted the development of DNA nanotechnology as it can construct delicate 2D/3D nanostructures. The current method to prepare DNA origami is through a woven approach to fix a long strand of DNA into certain shapes. This article describes a novel strategy to fold the same 2D DNA sheet into multiple complex structures driven by introducing hydrophobic interaction. The pathway of the folding process can be adjusted by tuning the distribution and chronic order of cholesterol, resulting in different complicated structures. This method is proven efficient for constructing variable nanostructures based on the same DNA origami sheet, and termed as origami(+). This work represents a new strategy in DNA nanotechnology and will improve the ability to manipulate objects at nanoscale.