Cholinergic regulation of proliferation of the urothelium in response to E. coli lipopolysaccharide exposition

被引:4
|
作者
Podmolikova, L. [1 ,3 ]
Mukanyangezi, M. F. [1 ]
Nieto-Marin, P. [1 ]
Giglio, D. [1 ,2 ]
机构
[1] Univ Gothenburg, Dept Pharmacol, BOX 431, SE-40530 Gothenburg, Sweden
[2] Univ Gothenburg, Dept Oncol, Gothenburg, Sweden
[3] Charles Univ Prague, Fac Med, Hradec Kralove, Czech Republic
关键词
ESCHERICHIA-COLI; RECEPTOR; 4; BLADDER; APOPTOSIS; CELLS; PHARMACOLOGY; IRRADIATION; INVOLVEMENT; EXPRESSION; INDUCTION;
D O I
10.1016/j.intimp.2018.01.006
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
How the proliferation of the urothelium is regulated is known to a little degree. E. coli lipopolysaccharide (LPS) activates the innate immune response of the urinary bladder via the Toll-like receptor 4 (TLR4) on the urothelium but induces also urothelial proliferation. We wanted to assess whether muscarinic receptors are involved in the regulation of urothelial proliferation triggered by LPS stimulation. Female Fischer 344 rats were instilled with LPS or saline (control) in the urinary bladder in the absence or presence of muscarinic receptor blockade with atropine and regeneration of the urothelium was assessed 4 h and 24 h later. In the Fischer 344 bladder, urothelial thinning and urothelial caspase 3 up-regulation occurred at 4 h after LPS urinary bladder instillation, which were totally blocked in rats pre-treated with atropine. TLR4 was only expressed in blood vessels in the Fischer 344 bladder, while it was also expressed in umbrella cells in the Sprague-Dawley bladder. Proliferation (Ki67 incorporation) of the human urothelial cell line UROtsa was reduced in the presence of the muscarinic receptor antagonists methoctramine (M2/M4-selective) and pirenzepine (M1/M4-selective), while proliferation instead was enhanced in the presence of atropine. In UROtsa cells exposed to LPS for 24 h, 4-DAMP (M3/M1/M5-selective) inhibited instead proliferation. In conclusion, muscarinic receptors regulate urothelial proliferation and LPS may induce urothelial apoptosis via muscarinic receptor-dependent pathways. Our findings also suggest that species differences exist in the expressional pattern of TLR4 in the urothelium.
引用
收藏
页码:222 / 229
页数:8
相关论文
共 50 条
  • [1] Determination of mouse bladder inflammatory response to E. coli lipopolysaccharide
    T. J. Jerde
    D. E. Bjorling
    H. Steinberg
    T. Warner
    R. Saban
    [J]. Urological Research, 2000, 28 : 269 - 273
  • [2] EFFECT OF E. COLI LIPOPOLYSACCHARIDE ON FEXOFENADINE PHARMACOKINETICS
    Jaisue, Siriluk
    Gerber, Jacobus P.
    McKinnon, Ross
    Davey, Andrew K.
    [J]. DRUG METABOLISM REVIEWS, 2008, 40 : 79 - 79
  • [3] Chlorpyriphos induces lung inflammation and alters response to E. coli lipopolysaccharide challenge
    Chougule, Amit Ajit
    Sethi, Ramsaran
    Schneberger, David
    Brar, Rajinder Singh
    Gill, Jatinder Paul Singh
    Singh, Baljit
    [J]. FASEB JOURNAL, 2013, 27
  • [4] PITing it forward: A new link in the journey of uropathogenic E. coli in the urothelium
    Joshi, Chetanchandra S.
    Cegelski, Lynette
    Mysorekar, Indira U.
    [J]. CELL REPORTS, 2022, 39 (04):
  • [5] Molecular Dynamics Simulations of E. coli Lipopolysaccharide Bilayers
    Wu, Emilia L.
    Engstrom, Olof
    Jo, Sunhwan
    Stuhlsatz, Dani
    Wildmalm, Goran
    Im, Wonpil
    [J]. BIOPHYSICAL JOURNAL, 2013, 104 (02) : 586A - 586A
  • [6] Multitier regulation of the E. coli extreme acid stress response by CsrA
    Gorelik, Mark G.
    Yakhnin, Helen
    Pannuri, Archana
    Walker, Alyssa C.
    Pourciau, Christine
    Czyz, Daniel
    Romeo, Tony
    Babitzke, Paul
    [J]. JOURNAL OF BACTERIOLOGY, 2024, 206 (04)
  • [7] Regulation of mRNA decay in E. coli
    Mohanty, Bijoy K.
    Kushner, Sidney R.
    [J]. CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2022, 57 (01) : 48 - 72
  • [8] E. coli Lipopolysaccharide: Acute oral toxicity study in mice
    Harper, Marc S.
    Carpenter, Carol
    Klocke, D. J.
    Carlson, Gabrielle
    Davis, Tom
    Delaney, Bryan
    [J]. FOOD AND CHEMICAL TOXICOLOGY, 2011, 49 (08) : 1770 - 1772
  • [9] Membrane rigidity regulates E. coli proliferation rates
    Salinas-Almaguer, Samuel
    Mell, Michael
    Almendro-Vedia, Victor G.
    Calero, Macarena
    Robledo-Sanchez, Kevin Carlo Martin
    Ruiz-Suarez, Carlos
    Alarcon, Tomas
    Barrio, Rafael A.
    Hernandez-Machado, Aurora
    Monroy, Francisco
    [J]. SCIENTIFIC REPORTS, 2022, 12 (01)
  • [10] Membrane rigidity regulates E. coli proliferation rates
    Samuel Salinas-Almaguer
    Michael Mell
    Victor G. Almendro-Vedia
    Macarena Calero
    Kevin Carlo Martín Robledo-Sánchez
    Carlos Ruiz-Suarez
    Tomás Alarcón
    Rafael A. Barrio
    Aurora Hernández-Machado
    Francisco Monroy
    [J]. Scientific Reports, 12