On the GFP-Based Analysis of Dynamic Concentration Profiles

被引：6
|
作者：
Berezhkovskii, Alexander M. [1 ]
Shvartsman, Stanislav Y. [2 ,3 ]
机构：
[1] NIH, Ctr Informat Technol, Div Computat Biosci, Math & Stat Comp Lab, Bethesda, MD 20892 USA
[2] Princeton Univ, Dept Chem & Biol Engn, Princeton, NJ 08544 USA
[3] Princeton Univ, Lewis Sigler Inst Integrat Genom, Princeton, NJ 08544 USA
来源：
BIOPHYSICAL JOURNAL | 2014年 / 106卷 / 03期
关键词：
D O I：
10.1016/j.bpj.2013.12.007
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Studies with GFP-tagged proteins can be used to investigate the dynamics of concentration profiles of regulatory proteins in cells and tissues. Analysis of these experiments must account for the finite rate with which the GFP-tagged proteins mature to the fluorescent state. Toward this end, we present an analytical framework that provides an explicit connection between the apparent kinetics of concentration gradients and the rates of GFP maturation.
引用
收藏
页码：L13 / L15
页数：3
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