A set of highly discriminating microsatellite loci for the Galapagos marine iguana Amblyrhynchus cristatus

被引:6
|
作者
Steinfartz, Sebastian
Caccone, Adalgisa
机构
[1] Yale Univ, Dept Ecol & Evolutionary Biol, New Haven, CT 06511 USA
[2] Yale Univ, Yale Inst Biospher Studies, Mol Systemat & Conservat Genet Lab, New Haven, CT 06511 USA
来源
MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 03期
关键词
Amblyrhynchus cristatus; Bayesian cluster analysis; Galapagos marine iguana; island differentiation; microsatellite loci;
D O I
10.1111/j.1471-8286.2006.01402.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We describe here the cloning of 12 (7 dinucleotide, 1 trinucleotide and 4 tetranucleotide) microsatellite loci for the Galapagos marine iguana Amblyrhynchus cristatus. When tested for individuals from five different island populations on the Galapagos archipelago, high genetic diversities (9-20 alleles per locus) and heterozygosities (0.200-0.944) were observed. All loci showed no obvious deviations from Hardy-Weinberg equilibrium. The new set of microsatellite loci was able to assign individuals reliably to their island of origin, thus being able to discriminate between residents and migrants between islands.
引用
收藏
页码:927 / 929
页数:3
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