Activation of peroxidase-catalyzed oxidation of 3,3′,5,5′-tetramethylbenzidine with poly(salicylic acid 5-aminodisulfide)

被引：7

作者：

Metelidza, DI ^{[1
]}

Karasyova, EI ^{[1
]}

机构：

[1] Natl Acad Sci Belarus, Inst Bioorgan Chem, Minsk 220141, BELARUS

来源：

BIOCHEMISTRY-MOSCOW | 2002年 / 67卷 / 09期

关键词：

horseradish peroxidase; tetramethylbenzidine; 5-aminosalicylic acid; poly(salicylic acid 5-aminodisulfide); activation; co-oxidation; inhibition of peroxidase-dependent reactions;

D O I：

10.1023/A:1020586305753

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

5-Aminosalicylic acid (5-ASA) inhibited by a mixed mechanism the peroxidase catalyzed oxidation of tetramethylbenzidine (TMB) in 0.015 M phosphate-citrate buffer (pH 6.4) supplemented with 5916 DMSO and 5% DMF. Poly(salicylic acid 5-aminodisulfide) (poly(SAADS)) in 0.01 M phosphate buffer (pH 6.2-7.4) supplemented with 5% DMSO and 5% DMF effectively activated the peroxidase-catalyzed oxidation of TMB. The activation was quantitatively characterized by coefficients alpha (M-1) determined at different pH values: a increased linearly with increase in pH up to the maximal value of 2.44.10(5) M-1 at pH 7.0. The activating effect of poly(SAADS) on the peroxidase-catalyzed oxidation of TMB is explained by the activator properties of polyelectrolyte, with its anionic form interacting with peroxidase sites responsible for the acid-base catalysis.

引用

页码：1048 / 1054

页数：7

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