Cell-Cycle-Dependent PC-PLC Regulation by APC/CCdc20-Mediated Ubiquitin-Proteasome Pathway

被引：21

作者：

Fu, Da ^{[1
]}

Ma, Yushui ^{[2
]}

Wu, Wei ^{[1
]}

Zhu, Xuchao ^{[1
]}

Jia, Chengyou ^{[1
]}

Zhao, Qianlei ^{[1
]}

Zhang, Chunyi ^{[1
]}

Wu, Xing Zhong ^{[1
]}

机构：

[1] Fudan Univ, Shanghai Med Coll, Dept Biochem, Shanghai 200032, Peoples R China

[2] Yangzhou Univ, Coll Vet Med, Dept Vet Clin Sci, Yangzhou 225009, Peoples R China

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2009年 / 107卷 / 04期

关键词：

Cdc20; PC-PLC; APC/C; CELL-CYCLE; UPP; ANAPHASE-PROMOTING COMPLEX; PROTEIN-KINASE-C; HYDROLYZING PHOSPHOLIPASE-C; FACTOR-KAPPA-B; SPINDLE CHECKPOINT; TRANSFORMED PHENOTYPE; FUNCTIONAL-ROLE; EARLY MITOSIS; CANCER-CELLS; ACTIVATION;

D O I：

10.1002/jcb.22163

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphatidylcholine-specific phospholipase C (PC-PLC) is involved in the cell signal transduction, cell proliferation, and apoptosis. The mechanism of its action, however, has not been fully understood, particularly, the role of PC-PLC in the cell cycle. In the present study, we found that cell division cycle 20 homolog (Cdc20) and PC-PLC were co-immunoprecipitated reciprocally by either antibody in rat hepatoma cells CBRH-7919 as well as in rat liver tissue. Using confocal microscopy, we found that PC-PLC and Cdc20 were co-localized in the perinuclear endoplasmic reticulum region (the "juxtanuclear quality control" compartment, JUNQ). The expression level and activities of PC-PLC changed in a cell-cycle-dependent manner and were inversely correlated with the expression of Cdc20. Intriguingly, Cdc20 overexpression altered the subcellular localization and distribution of PC-PLC, and caused PC-PLC degradation by the ubiquitin proteasome pathway (UPP). Taken together, our data indicate that PC-PLC regulation in cell cycles is controlled by APC/C-Cdc20-mediated UPP. J. Cell. Biochem. 107: 686-696, 2009. (C) 2009 Wiley-Liss, Inc.

引用

页码：686 / 696

页数：11

共 47 条

[1] Cell cycle-dependent regulation of the ubiquitin-proteasome pathway (UPP)
Shang, F
Liu, Q
Hobbs, M
Taylor, A
FASEB JOURNAL, 2003, 17 (05): : A1006 - A1007
[2] Regulation of the endothelial cell cycle by the ubiquitin-proteasome system
Fasanaro, Pasquale
Capogrossi, Maurizio C.
Martelli, Fabio
CARDIOVASCULAR RESEARCH, 2010, 85 (02) : 272 - 280
[3] Cksl is degraded via the ubiquitin-proteasome pathway in a cell cycle-dependent manner
Hattori, T
Kitagawa, K
Uchida, C
Oda, T
Kitagawa, M
GENES TO CELLS, 2003, 8 (11) : 889 - 896
[4] The ubiquitin-proteasome pathway regulates survivin degradation in a cell cycle-dependent manner
Zhao, J
Tenev, T
Martins, LM
Downward, J
Lemoine, NR
JOURNAL OF CELL SCIENCE, 2000, 113 (23) : 4363 - 4371
[5] Regulation of the ubiquitin-proteasome pathway (UPP) during lens cell differentiation
Guo, W
Shang, F
Liu, Q
Taylor, A
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2003, 44 : U261 - U261
[6] Regulation of follitropin receptor cell surface residency by the ubiquitin-proteasome pathway
Cohen, BD
Bariteau, JT
Magenis, LM
Dias, JA
ENDOCRINOLOGY, 2003, 144 (10) : 4393 - 4402
[7] Differential regulation of components of the ubiquitin-proteasome pathway during lens cell differentiation
Guo, WM
Shang, F
Liu, Q
Urim, L
West-Mays, J
Taylor, A
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2004, 45 (04) : 1194 - 1201
[8] Functional roles of PC-PLC and Cdc20 in the cell cycle, proliferation, and apoptosis
Chen, Zhiwei
Yu, Yongfeng
Fu, Da
Li, Ziming
Niu, Xiaoming
Liao, Meilin
Lu, Shun
CELL BIOCHEMISTRY AND FUNCTION, 2010, 28 (04) : 249 - 257
[9] Ubiquitin-proteasome pathway as a primary defender against TRAIL-mediated cell death
S. Kim
K. Choi
D. Kwon
E. N. Benveniste
C. Choi
Cellular and Molecular Life Sciences CMLS, 2004, 61 : 1075 - 1081
[10] Ubiquitin-proteasome pathway as a primary defender against TRAIL-mediated cell death
Kim, S
Choi, K
Kwon, D
Benveniste, EN
Choi, C
CELLULAR AND MOLECULAR LIFE SCIENCES, 2004, 61 (09) : 1075 - 1081

← 1 2 3 4 5 →