Fungal secretomes enhance sugar beet pulp hydrolysis

被引:18
|
作者
Kracher, Daniel [1 ]
Oros, Damir [2 ]
Yao, Wanying [1 ]
Preims, Marita [1 ]
Rezic, Iva [3 ]
Haltrich, Dietmar [1 ]
Rezic, Tonci [2 ]
Ludwig, Roland [1 ]
机构
[1] BOKU Univ Nat Resources & Life Sci, Dept Food Sci & Technol, Food Biotechnol Lab, Vienna, Austria
[2] Univ Zagreb, Fac Food Technol & Biotechnol, Dept Biochem Engn, Zagreb 41000, Croatia
[3] Univ Zagreb, Fac Text Technol, Dept Appl Chem, Zagreb 41000, Croatia
基金
奥地利科学基金会;
关键词
Cellulases; Hemicellulases; Neurospora crassa; Sclerotium rolfsii; Trametes multicolor; MODEL FILAMENTOUS FUNGUS; CELLOBIOSE DEHYDROGENASE; FUEL ETHANOL; ENZYMATIC SACCHARIFICATION; CELLULOSE DEGRADATION; DEGRADING ENZYMES; ACID; FERMENTATION; BIOMASS;
D O I
10.1002/biot.201300214
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The recalcitrance of lignocellulose makes enzymatic hydrolysis of plant biomass for the production of second generation biofuels a major challenge. This work investigates an efficient and economic approach for the enzymatic hydrolysis of sugar beet pulp (SBP), which is a difficult to degrade, hemicellulose-rich by-product of the table sugar industry. Three fungal strains were grown on different substrates and the production of various extracellular hydrolytic and oxidative enzymes involved in pectin, hemicellulose, and cellulose breakdown were monitored. In a second step, the ability of the culture supernatants to hydrolyze thermally pretreated SBP was tested in batch experiments. The supernatant of Sclerotium rolfsii, a soil-borne facultative plant pathogen, was found to have the highest hydrolytic activity on SBP and was selected for further hydrolyzation experiments. A low enzyme load of 0.2 mg g(-1) protein from the culture supernatant was sufficient to hydrolyze a large fraction of the pectin and hemicelluloses present in SBP. The addition of Trichoderma reesei cellulase (1-17.5 mg g(-1) SBP) resulted in almost complete hydrolyzation of cellulose. It was found that the combination of pectinolytic, hemicellulolytic, and cellulolytic activities works synergistically on the complex SBP composite, and a combination of these hydrolytic enzymes is required to achieve a high degree of enzymatic SBP hydrolysis with a low enzyme load.
引用
收藏
页码:483 / 492
页数:10
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