The mitochondrial phospholipid cardiolipin is synthesized from cytidinediphosphate-diacylglycerol and phosphatidylglycerol, a process catalyzed by the enzyme cardiolipin synthase. In this study, we identified a human candidate gene/cDNA for cardiolipin synthase, C20orf155. Expression of this candidate cDNA in the (cardiolipin synthase-deficient) crd1 Delta yeast confirmed that it indeed encodes human cardiolipin synthase. Purified mitochondria of the crd1 Delta expressing human cardiolipin synthase were used to characterize the enzyme. It has an alkaline pH optimum, requires divalent cations for activity and appears to have a different substrate preference for cytidinediphosphate-diacylglycerol species when compared to phosphatidylglycerol species. The possible implications for CL synthesis and remodeling are discussed. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
机构:
Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Tokyo, JapanAcad Sinica, Inst Plant & Microbial Biol, Taipei, Taiwan
Kobayashi, Koichi
Wada, Hajime
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机构:
Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Tokyo, Japan
CREST, Japan Sci & Technol Agcy, Saitama, JapanAcad Sinica, Inst Plant & Microbial Biol, Taipei, Taiwan