Identification and characterization of human cardiolipin synthase

被引:77
|
作者
Houtkooper, Riekelt H.
Akbari, Hana
van Lenthe, Henk
Kulik, Willem
Wanders, Ronald J. A.
Frentzen, Margrit
Vaz, Frederic M.
机构
[1] Univ Amsterdam, Acad Med Ctr, Lab Genet Metab Dis, Dept Clin Chem, NL-1100 DE Amsterdam, Netherlands
[2] Univ Amsterdam, Acad Med Ctr, Lab Genet Metab Dis, Dept Pediat, NL-1100 DE Amsterdam, Netherlands
[3] Rhein Westfal TH Aachen, Inst Biol 1, D-52056 Aachen, Germany
来源
FEBS LETTERS | 2006年 / 580卷 / 13期
关键词
cardiolipin synthase; phosphatidylglycerol; CDP-diacylglycerol; substrate specificity;
D O I
10.1016/j.febslet.2006.04.054
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The mitochondrial phospholipid cardiolipin is synthesized from cytidinediphosphate-diacylglycerol and phosphatidylglycerol, a process catalyzed by the enzyme cardiolipin synthase. In this study, we identified a human candidate gene/cDNA for cardiolipin synthase, C20orf155. Expression of this candidate cDNA in the (cardiolipin synthase-deficient) crd1 Delta yeast confirmed that it indeed encodes human cardiolipin synthase. Purified mitochondria of the crd1 Delta expressing human cardiolipin synthase were used to characterize the enzyme. It has an alkaline pH optimum, requires divalent cations for activity and appears to have a different substrate preference for cytidinediphosphate-diacylglycerol species when compared to phosphatidylglycerol species. The possible implications for CL synthesis and remodeling are discussed. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:3059 / 3064
页数:6
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