3' non-templated 'A' addition by Taq DNA polymerase: An advantage in the construction of single and double mutants

被引：0
|
作者：
JagathReddy, J
Rao, NA
Savithri, HS
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来源：
CURRENT SCIENCE | 1996年 / 71卷 / 09期
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中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The 3'-end non-templated 'A' addition by Tag DNA polymerase has been described as a disadvantage in the generation of site-specific mutants as this 'A' leads to a unplanned second mutation, Here we demonstrate the utility of this 3' non-templated 'A' addition in the simultaneous construction of single and double mutants of serine hydroxymethyltransferase.
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页码：710 / 712
页数：3
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