Phosphoenolpyruvate carboxylase of maize leaves: An improved method for purification and reduction of the inhibitory effect of malate by ethylene glycol and bicarbonate

被引:16
|
作者
Ogawa, N [1 ]
Kai, T [1 ]
Yabuta, N [1 ]
Izui, K [1 ]
机构
[1] KYOTO UNIV,FAC AGR,DEPT AGR BIOL,KYOTO 60601,JAPAN
关键词
C-4; photosynthesis; ethylene glycol; malate inhibition; phosphoenolpyruvate carboxylase (EC 4.1.1.31); purification; Zea mays;
D O I
10.1093/oxfordjournals.pcp.a029088
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Light-adapted and dark-adapted forms of phosphoenolpyruvate carboxylase were purified from maize leaves by an improved method that included chromatography on Butyl-Toyopearl in the presence of ethylene glycol. The inhibition by malate was relieved not only by increasing concentrations of ethylene glycol but also by bicarbonate at pH 7.0.
引用
收藏
页码:76 / 80
页数:5
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