Minimal Residual Disease Detection by Next-Generation Sequencing in Multiple Myeloma: A Comparison With Real-Time Quantitative PCR

被引：2

作者：

Yao, Qiumei ^{[1
]}

Bai, Yinlei ^{[2
,3
]}

Kumar, Shaji ^{[4
]}

Au, Elaine ^{[5
]}

Orfao, Alberto ^{[6
,7
,8
]}

Chim, Chor Sang ^{[1
]}

机构：

[1] Univ Hong Kong, Dept Med, Pokfulam, Queen Mary Hosp, Hong Kong, Peoples R China

[2] Tsinghua Univ, Inst Immunol, Beijing, Peoples R China

[3] Tsinghua Univ, Sch Med, Beijing, Peoples R China

[4] Mayo Clin, Div Hematol, Rochester, MN USA

[5] Univ Hong Kong, Dept Pathol, Pokfulam, Queen Mary Hosp, Hong Kong, Peoples R China

[6] Univ Salamanca USAL, Dept Med, Salamanca, Spain

[7] Univ Salamanca USAL, Cytometry Serv Nucleus, Canc Res Ctr IBMCC, USAL CSIC, Salamanca, Spain

[8] Univ Salamanca USAL, CIBERONC, Inst Biomed Res Salamanca IBSAL, Salamanca, Spain

来源：

FRONTIERS IN ONCOLOGY | 2021年 / 10卷

基金：

中国国家自然科学基金;

关键词：

minimal residual disease; multiple myeloma; next-generation sequencing; allele-specific oligonucleotide real-time quantitative-PCR; sensitivity;

D O I：

10.3389/fonc.2020.611021

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Here we compared clonotype identification by allele-specific oligonucleotide real-time quantitative-PCR (ASO RQ-PCR) and next-generation sequencing (NGS) in 80 multiple myeloma patients. ASO RQ-PCR was applicable in 49/55 (89%) and NGS in 62/78 (80%). Clonotypes identified by both methods were identical in 33/35 (94%). Sensitivity of 10(-5) was confirmed in 28/29 (96%) by NGS while sensitivity of RQ-PCR was 10(-5) in 7 (24%), 5 x 10(-5) in 15 (52%), and 10(-4) in 7 (24%). Among 14 samples quantifiable by ASO RQ-PCR, NGS yielded comparable results in 12 (86%). Applicability of NGS can be improved if immunoglobulin heavy-chain incomplete DJ primers are included.

引用

页数：5

共 50 条

[1] Minimal Residual Disease (MRD) detection by next-generation sequencing and real-time quantitative PCR: A methodical comparison in ALL, MCL and MM
Brueggemann, M.
Ladetto, M.
Monitillo, L.
Ferrero, S.
Pepin, F.
Drandi, D.
Palumbo, A.
Boccadero, M.
Carlton, V
Trautmann, H.
Kneba, M.
Faham, M.
Ritgen, M.
Pott, C.
[J]. ONKOLOGIE, 2013, 36 : 169 - 169
[2] MINIMAL RESIDUAL DISEASE (MRD) DETECTION BY NEXT-GENERATION SEQUENCING AND REAL-TIME QUANTITATIVE PCR: A METHODICAL COMPARISON IN ALL, MCL AND MM
Ladetto, M.
Brueggemann, M.
Monitillo, L.
Ferrero, S.
Pepin, F.
Drandi, D.
Palumbo, A.
Passera, R.
Boccadoro, M.
Carlton, V.
Trautmann, H.
Faham, M.
Pott, C.
[J]. HAEMATOLOGICA, 2013, 98 : 125 - 125
[3] A COMPARATIVE ANALYSIS OF NEXT-GENERATION SEQUENCING AND REAL-TIME QUANTITATIVE PCR FOR MINIMAL RESIDUAL DISEASE DETECTION IN FOLLICULAR LYMPHOMA
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Monitillo, L.
Genuardi, E.
Mantoan, B.
Trautmann, H.
Kneba, M.
Brueggemann, M.
Faham, M.
Ladetto, M.
[J]. HAEMATOLOGICA, 2013, 98 : 124 - 125
[4] Next-generation sequencing and real-time quantitative PCR for minimal residual disease detection in B-cell disorders
M Ladetto
M Brüggemann
L Monitillo
S Ferrero
F Pepin
D Drandi
D Barbero
A Palumbo
R Passera
M Boccadoro
M Ritgen
N Gökbuget
J Zheng
V Carlton
H Trautmann
M Faham
C Pott
[J]. Leukemia, 2014, 28 : 1299 - 1307
[5] Next-generation sequencing and real-time quantitative PCR for minimal residual disease detection in B-cell disorders
Ladetto, M.
Brueggemann, M.
Monitillo, L.
Ferrero, S.
Pepin, F.
Drandi, D.
Barbero, D.
Palumbo, A.
Passera, R.
Boccadoro, M.
Ritgen, M.
Goekbuget, N.
Zheng, J.
Carlton, V.
Trautmann, H.
Faham, M.
Pott, C.
[J]. LEUKEMIA, 2014, 28 (06) : 1299 - 1307
[6] Standardized Minimal Residual Disease Detection by Next-Generation Sequencing in Multiple Myeloma
Yao, Qiumei
Bai, Yinlei
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[J]. FRONTIERS IN ONCOLOGY, 2019, 9
[7] Upgraded Standardized Minimal Residual Disease Detection by Next-Generation Sequencing in Multiple Myeloma
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[J]. JOURNAL OF MOLECULAR DIAGNOSTICS, 2020, 22 (05): : 679 - 684
[8] A comparison between next-generation sequencing and ASO-qPCR for minimal residual disease detection in multiple myeloma
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Murata, Ryoichi
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[J]. JOURNAL OF CLINICAL ONCOLOGY, 2013, 31 (15)
[9] A COMPARISON BETWEEN NEXT-GENERATION SEQUENCING AND ASO-QPCR FOR MINIMAL RESIDUAL DISEASE DETECTION IN MULTIPLE MYELOMA
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[J]. HAEMATOLOGICA, 2013, 98 : 101 - 102
[10] A comparative analysis of next-generation sequencing and t(14;18) real-time quantitative PCR for Minimal Residual Disease Detection (MRD) in Follicular lymphoma
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[J]. ONKOLOGIE, 2013, 36 : 31 - 31

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