An Sp1 binding site is essential for basal activity of the human prostate-specific transglutaminase gene (TGM4) promoter

被引：11

作者：

Dubbink, HJ

Cleutjens, KBJM

van der Korput, HAGM

Trapman, J

Romijn, JC

机构：

[1] Erasmus Univ, Dept Urol, Josephine Nefkens Inst, NL-3000 DR Rotterdam, Netherlands

[2] Erasmus Univ, Dept Pathol, Josephine Nefkens Inst, NL-3000 DR Rotterdam, Netherlands

来源：

GENE | 1999年 / 240卷 / 02期

关键词：

core promoter; Sp1; Sp3; TGase; TG(p); transcription regulation;

D O I：

10.1016/S0378-1119(99)00454-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Human prostate-specific transglutaminase (hTG(P)) is a cross-linking enzyme encoded by the TGM4 gene. The TGM4 gene promoter was characterized by deletion mapping and mutational analysis. Promoter constructs, containing the minimal promoter requirements, could efficiently drive transcription in the prostate cancer cell lines PC346C and LNCaP and the hepatic cancer cell line Hep3B. The region between positions -113 and -61 was demonstrated to be essential for core promoter activity. Further analysis revealed the functional importance of an Spl binding motif, 5'-ACCCCGCCCC-3', at positions -96 to -87. This sequence is a binding site of the ubiquitous transcription factors Spl and Sp3. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：261 / 267

页数：7

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