MicroRNA-147b Promotes Proliferation and Invasion of Human Colorectal Cancer by Targeting RAS Oncogene Family (RAP2B)

被引:17
|
作者
Yi, Lizhi [1 ]
Zhong, Xianfei [1 ]
Chen, Zhengyu [1 ]
Wang, Qin [1 ]
Yan, Yongrong [3 ]
Wang, Jianmei [2 ]
Deng, Xuejie [1 ]
机构
[1] Peoples Hosp Leshan, Dept Gastroenterol, 238 Baita St, Leshan City 614000, Sichuan, Peoples R China
[2] Southwest Med Univ, Affiliated Hosp, Dept Pathol, 25 Taiping St, Luzhou 646000, Sichuan, Peoples R China
[3] Southern Med Univ, Nanfang Hosp, Dept Pathol, Guangzhou, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
miR-147b; Proliferation; Invasion; Colorectal cancer; RAP2B; MIGRATION; BIOMARKER; MIR-147B;
D O I
10.1159/000495253
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Background: MicroRNAs (miRNAs), a class of small-regulatory RNA molecules, were closely involved in the pathogenesis of a broad-spectrum of colorectal cancer (CRC). But role of miR-147b in CRC still remains unclear. Methods: Real-time RT-PCR or Western blotting was utilized to detect the expressions of miR-147b and RAP2B in CRC cell lines and tissues. Luciferase reporter assays were conducted to detect the associations between miR-147b and 3 ' UTRs of RAP2B. A series of assays were performed to evaluate the effect of miR-147b on proliferation, migration, and invasion of CRC in vitro and in vivo. Results: We found that the level of miR-147b was significantly lower in CRC tissues than in normal tissues (p = 0.0006). Enforced expression of miR-147b led to suppression of CRC cell proliferation in vitro and tumor growth in vivo. Specifically, miR-147b promoted proliferation by arresting CRC cells in the G1/G0 phase. Mechanically, RAP2B was identified as a direct target gene of miR-147b and RAP2B rescued the suppression of proliferation and invasion reduced by miR-147b in CRC cells. Conclusions: miR-147b not only plays important roles in the regulation of cell proliferation and tumor growth in CRC, which might be a potential prognostic marker or therapeutic target for CRC.
引用
收藏
页码:173 / 181
页数:9
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