A polymyxin-cloth enzyme immunoassay system for detection of Escherichia coli O157 in ground beef

被引：2
|
作者：
Booth, RA
Blais, BW
Johnson, RP
Yamazaki, H
机构：
[1] AGR & AGRI FOOD CANADA,FOOD PROD & INSPECT BRANCH,LAB SERV DIV,OTTAWA,ON K1A 0C6,CANADA
[2] CARLETON UNIV,DEPT BIOL,OTTAWA,ON K1S 5B6,CANADA
[3] CARLETON UNIV,INST BIOCHEM,OTTAWA,ON K1S 5B6,CANADA
[4] AGR & AGRI FOOD CANADA,FOOD PROD & INSPECT BRANCH,HLTH ANIM LAB,GUELPH,ON N1G 3W4,CANADA
来源：
FOOD MICROBIOLOGY | 1996年 / 13卷 / 03期
基金：
加拿大自然科学与工程研究理事会;
关键词：
D O I：
10.1006/fmic.1996.0029
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A polymyxin-cloth enzyme immunoassay (polymyxin-CEIA) was developed for the detection of the foodborne pathogen Escherichia coli O157. This simple assay utilized inexpensive polymyxin-coated polyester cloth as an adsorbent for E. coli lipopolysaccharide (LPS) antigens present in test samples such as enrichment cultures of ground beef. Captured LPS was immunoenzymatically defected using commercially available anti-O157 polyclonal antibodies. The assay was sensitive and highly specific for strains bearing the O157 and related antigens, and permitted the detection of c. 1-10 cfus spiked into ground beef samples after a brief enrichment in double modified trypticase soy broth. We suggest that the polymyxin-CEIA will provide a simple, rapid and inexpensive screening method for E. coli O157 in ground beef. (C) 1996 Academic Press Limited
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页码：237 / 242
页数：6
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