Effects of molecular orientation of fluorescently-labeled duplex DNA on emission anisotropy

被引：1
|
作者：
Sato, Akihiro [1 ]
Menges, Bernhard [1 ]
Knoll, Wolfgang [1 ]
机构：
[1] Max Planck Inst Polymer Res, Mat Sci Croup, D-55128 Mainz, Germany
来源：
OPTICS COMMUNICATIONS | 2009年 / 282卷 / 05期
关键词：
Orientation; Fluorescence spectroscopy; Optical waveguide; OPTICAL WAVE-GUIDE; GOLD SURFACES; THIN-FILMS; MONOLAYERS; SPECTROSCOPY; DENSITY; HYBRIDIZATION; MEMBRANES;
D O I：
10.1016/j.optcom.2008.11.065
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
The molecular orientation of Cy5-labeled target DNA hybridized on a probe DNA layer was studied on a planar waveguide in the Kretchmarm configuration at various target DNA bulk concentration. Optical waveguide fluorescence spectroscopy (OWFS) was employed to evaluate the fluorescence emission anisotropy by analyzing two polarized modes, i.e.. TM and TE modes propagating in a waveguide. In this way, the orientational order parameter S could be determined. It was found that the orientation of DNA duplex molecules is disordered at lower concentrations, while relatively ordered at higher concentrations. (C) 2008 Elsevier B.V. All rights reserved.
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页码：1062 / 1066
页数：5
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