DETERMINATION OF IN VITRO FREE RADICAL SCAVENGING ACTIVITIES OF VARIOUS EXTRACTS FROM IN VITRO PROPAGATED Ceratophyllum demersum L.

The study presents multiple shoot regeneration of Ceratophyllum demersum L. from shoot tip meristem explants culturing on liquid MS medium 0.05-0.40 mg/L Kinetin (KIN). Thereafter, free radical scavenging activities with 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) of aqueous and methanol extracts of C. demersum were also investigated. The highest number of shoots per explants (74.53) and the highest shoot length (4.93 cm) were obtained on MS medium containing 0.20 mg/L Kinetin. Extracts from C. demersum were effective in the following order: water > methanol. DPPH scavenging activities of treatments with maximum concentration (10 mg/ml) of water and methanol extracts were 68.91 and 51.22%, respectively. It was shown that there was high positive correlation between DPPH scavenging activity and concentration for both extracts. At the same time, IC50 values of water and methanol extracts showed aqueous as most effective extract with 3.689 mg/ml.