The Tyrosinase-Encoding Gene of Lentinula edodes, Letyr, Is Abundantly Expressed in the Gills of the Fruit-Body during Post-Harvest Preservation

被引：15

作者：

Sato, Toshitsugu ^{[1
]}

Kanda, Katsuhiro ^{[1
]}

Okawa, Kumiko ^{[1
]}

Takahashi, Machiko ^{[1
]}

Watanabe, Hisayuki ^{[1
]}

Hirano, Tatsuya ^{[1
]}

Yaegashi, Kaori ^{[1
]}

Sakamoto, Yuichi ^{[1
]}

Uchimiya, Hirofumi ^{[1
]}

机构：

[1] Iwate Biotechnol Res Ctr, Kitakami, Iwate 0240003, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2009年 / 73卷 / 05期

关键词：

Lentinula edodes; tyrosinase gene; transcription; fruit-body browning; post-harvest preservation; TRANSFORMATION; ELEMENT; CLONING; PLANT;

D O I：

10.1271/bbb.80810

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gill browning of Lentinula edodes fruit-bodies during preservation is thought to be due to melanin biosynthesis catalyzed by tyrosinase. We isolated a genomic DNA sequence and cDNA encoding a putative tyrosinase from the white rot basidiomycete Lentinula edodes (shiitake mushroom). The gene, named Letyr, consists of a 1,854-bp open reading frame interrupted by eight introns, and encodes a putative protein of 618 amino acid residues with an estimated molecular mass of 68 kDa. Amino acid residues known to be involved in copper-binding domains were conserved in the deduced amino acid residues of LeTyr. Transcriptional and translational expression of Letyr in the gills of the fruit-body increased during preservation after harvest. This correlation between Letyr expression and fruit-body preservation suggests that tyrosinase gene expression contributes to gill browning.

引用

页码：1042 / 1047

页数：6