PCR identification of Mycobacterium bovis BCG

被引：141
|
作者：
Talbot, EA
Williams, DL
Frothingham, R
机构：
[1] VET AFFAIRS MED CTR, INFECT DIS SECT, DURHAM, NC 27705 USA
[2] DUKE UNIV, MED CTR, DEPT MED, DIV INFECT DIS & INT HLTH, DURHAM, NC 27710 USA
[3] LOUISIANA STATE UNIV, GW LONG HANSENS DIS CTR, LAB RES BRANCH, MOL BIOL RES DEPT, BATON ROUGE, LA 70803 USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 03期
关键词：
D O I：
10.1128/JCM.35.3.566-569.1997
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The attenuated bacillus Calmette-Guerin (BCG) vaccine strain is derived from a virulent strain of Mycobacterium bovis. BCG is difficult to differentiate from other strains of M. bovis and other members of the M. tuberculosis complex by conventional methods. Recently, a genomic region designated RD1 was found to be present in all virulent M. bovis and M. tuberculosis strains tested but deleted from all BCG strains tested. With this information, a multiplex PCR method was developed to detect the RD1 deletion. A large collection of BCG and other M. tuberculosis complex strains from diverse host and geographic origins was tested. RD1 was deleted in 23 of 23 BCG strains. RD1 was present in 129 of 129 other M. tuberculosis complex strains. This multiplex PCR method can be used as a tool for the rapid and specific identification of BCG.
引用
收藏
页码：566 / 569
页数：4
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