Engineering, differentiation and harvesting of human adipose-derived stem cell multilayer cell sheets

被引：14
|
作者：
Oliva, Joan [1 ,2 ]
Florentino, Arjie [1 ]
Bardag-Gorce, Fawzia [1 ]
Niihara, Yutaka [1 ,2 ]
机构：
[1] Harbor UCLA Med Ctr, Dept Med, LA BioMed, Torrance, CA 90509 USA
[2] Emmaus Life Sci Inc, 21250 Hawthorne Blvd,Suite 800, Torrance, CA 90503 USA
来源：
REGENERATIVE MEDICINE | 2019年 / 14卷 / 03期
关键词：
adipose-derived stem cells; cell therapy; differentiation; gene therapy; harvesting; multilayer cell sheet; BONE-MARROW; TRANSPLANTATION; SCAFFOLDS; THERAPY;
D O I：
10.2217/rme-2018-0053
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Aim: The study goals were to engineer and harvest scaffold-free undifferentiated/differentiated multilayer human adipose-derived stem cell (hADSC) cell sheets, in absence of treatment. Materials & methods: The hADSC are seeded in 35 mm culture dishes. At confluence or when multilayer cell sheets are formed, hADSC are treated with predefined differentiation culture media (adipocyte, chondrocyte and osteoblast). Results: Undifferentiated hADSC and differentiated adipocyte, osteoblast and chondrocyte hADSC multilayer cell sheets (hADSCmCS) have been harvested. Hematoxylin & eosin showed the formation of multilayer cell sheets. Undifferentiated hADSC multilayer cell sheets preserve their stem cell markers. Differentiated adipocyte, osteoblast and chondrocyte hADSCmCS expressed specific markers. Conclusion: This simple protocol opens possibilities to engineer scaffold-free hADSCm cell sheet to transplant them on damaged organs. [GRAPHICS] .
引用
收藏
页码：151 / 163
页数：13
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