Variation in leaf structures of micropropagated rhubarb (Rheum rhaponticum L.) PC49

被引：10

作者：

Zhao, Yipeng ^{[1
]}

Zhou, Yan

Grout, Brian W. W.

机构：

[1] Henan Inst Sci & Technol, Dept Hort, Xinxiang 453003, Henan, Peoples R China

[2] Henan Inst Sci & Technol, Dep Plant Sci & Technol, Xinxiang 453003, Henan, Peoples R China

[3] Writtle Coll, Postgrad Sch, Chelmsford CM1 3RR, Essex, England

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2006年 / 85卷 / 01期

关键词：

cellular structure; somaclonal variation; trichome; twin-stomata;

D O I：

10.1007/s11240-005-9053-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Alterations in leaf trichomes, stomatal characteristics and epidermal cellular features were investigated in micropropagated rhubarb (Rheum rhaponticum L.) PC49. The results showed that micropropagated regenerants had produced significantly lower stomatal index, but larger epidermal cell size than conventional plants. In addition, altered trichome morphology and abnormal stomata, e.g. twin-stomata were constantly noted only in micropropagated plants. The microscopic observation demonstrated a substantially larger intercellular space in palisade and mesophyll only in leaves of micropropagated plants. But the results showed no difference in chloroplast number and chlorophyll content between micropropagated and conventional plants. All the abnormalities suggest somaclonal variation may have occurred in micropropagated rhubarb PC49.

引用

页码：115 / 121

页数：7

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