Two kinds of three-dimensional spectroscopes were introduced including time-resolved spectroscopy and two-dimensional spectroscopy. Their theoretical basis and algorithm were described. The fundamental concepts of two-dimensional infrared spectroscopy was introduced as well as its applications in polymer, chemistry, biochemistry et al. Three-Dimensional Spectroscopy has been one of the most important methods for spectroscopes. The research on time-resolved spectroscopy and two-dimensional correlation spectroscopy has been done, including FTIR-TRS studies of the influence of PH on BR photocycle; studies of N phosphoryl Histidine and Isopropanol reaction system; studies on autosensitive photooxygenation of a new cancercuring medicine-hypocrellin; studies of crystal phase transformation in polyvinylidene fluoride; studies on self-activation of phosphorylated aminoacids to form peptides et al.