Optimization of polyethylene glycol (PEG)-mediated DNA introduction conditions for transient gene expression in the unicellular red alga Cyanidioschyzon merolae

被引：10
|
作者：
Ohnuma, Mio [1 ,2 ,3 ]
Yokoyama, Takashi [4 ]
Inouye, Takayuki [4 ]
Sekine, Yasuhiko [4 ]
Kuroiwa, Tsuneyoshi [2 ,3 ]
Tanaka, Kan [1 ,3 ,5 ]
机构：
[1] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan
[2] Rikkyo Univ, Coll Sci, Initiat Res Unit, Toshima Ku, Tokyo 1718501, Japan
[3] Japan Sci & Technol Agcy JST, CREST, Kawaguchi, Saitama 3320012, Japan
[4] Rikkyo St Pauls Univ, Coll Sci, Dept Life Sci, Tokyo 1718501, Japan
[5] Tokyo Inst Technol, Chem Resources Lab, Midori Ku, Yokohama, Kanagawa 2268503, Japan
来源：
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | 2014年 / 60卷 / 04期
基金：
日本科学技术振兴机构; 日本学术振兴会;
关键词：
Cyanidioschyzon merolae; DNA introduction; luciferase; polyethylene glycol; FIREFLY LUCIFERASE; STABLE EXPRESSION; REPORTER GENE; TRANSFORMATION; SEQUENCE; GENOME; 10D; PROTOPLASTS; CARRIER; UTILITY;
D O I：
10.2323/jgam.60.156
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
[No abstract available]
引用
收藏
页码：156 / 159
页数：4
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