In vitro propagation of Caralluma sarkariae Lavranos & Frandsen - An endemic and endangered medicinal plant

被引:0
|
作者
Sreelatha, V. Raja [1 ]
Rani, S. Sandhya [1 ]
Reddy, P. V. Krishna [1 ]
Naveen, M. [1 ]
Ugraiah, A. [1 ]
Pullaiah, T. [1 ]
机构
[1] Sri Krishnadevaraya Univ, Dept Bot, Anantapur 515003, Andhra Pradesh, India
来源
INDIAN JOURNAL OF BIOTECHNOLOGY | 2009年 / 8卷 / 02期
关键词
Callus cultures; Caralluma sarkariae; tissue culture; CEROPEGIA; MICROPROPAGATION;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An efficient protocol has been developed for in vitro propagation of Caralluma sarkariae Lavranos & Frandsen via mature internodal derived callus. Optimal callus was developed for regeneration front mature internodal explants on Murashige and Skoog (MS) basal medium supplemented with various concentrations of auxins. Maximum number of shoots were regenerated (65%) from the callus On MS medium supplemented with 6-benzyl aminopurine (BAP) 2.0 mg/L + kinetin (KN) 0.5 mg/L + naphthalene acetic acid (NAA) 0.5 mg/L. Individual elongated shoots were rooted on half strength MS medium containing NAA 0.1 mg/L. Regenerated plantlets with well developed shoots and roots Were successfully transferred to soil.
引用
收藏
页码:236 / 239
页数:4
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