Purification and characterization of milk-clotting enzymes from oyster mushroom (Pleurotus ostreatus (Fr.) Kumm)

被引：4
|
作者：
Lebedeva, G. V. [1 ]
Proskuryakov, M. T. [1 ]
机构：
[1] Kuban State Univ, Krasnodar 350040, Russia
来源：
APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2009年 / 45卷 / 06期
关键词：
Apply Biochemistry; Isoelectric Point; Fruiting Body; Pleurotus Ostreatus; Oyster Mushroom;
D O I：
10.1134/S0003683809060088
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Three enzymes with milk-clotting activity have been isolated from the fruiting bodies of Pleurotus ostreatus (Fr.) Kumm) by (NH4)(2)SO4 precipitation, gel chromatography on Sephadex G75, and ion exchange chromatography on carboxymethylcellulose (CMC). Isoelectric points of the enzymes, as determined by isoelectrofocusing, equaled 4.2, 6.7, and 8.8. Inhibition analysis showed that the enzymes with isoelectric points of 4.2 and 6.7 belong to the class of metal-dependent proteinases, while the enzyme with the isoelectric point of 8.8 belongs to the serine protease class.
引用
收藏
页码：623 / 625
页数：3
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