Expression parameters for target gene cloned in Escherichia coli in response to phosphate supply

被引：6

作者：

Bhattacharya, SK ^{[1
]}

Dubey, AK ^{[1
]}

机构：

[1] INDIAN INST TECHNOL,DEPT BIOCHEM ENGN & BIOTECHNOL,NEW DELHI 110016,INDIA

来源：

BIOTECHNOLOGY LETTERS | 1996年 / 18卷 / 10期

关键词：

D O I：

10.1007/BF00128582

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recombinant strain of E. coli 1727 overexpressed target gene at enhanced level when supplied with excess of inorganic phosphate. Rate of target gene expression, yield coefficient for target gene product and plasmid copy number increased significantly: 50%, 100% and 40% respectively at 125 mM excess phosphate. This was, however, accompanied by 26% decrease in specific growth rate and 30% in cellular growth yield coefficient.

引用

页码：1145 / 1148

页数：4

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