A Robust Method for Determination of Ochratoxin A in Wine Samples by SPE and HPLC-FLD

The aim of this work was the developing of a sensitive, accurate, reproducible and inexpensive HPLC with fluorescence detection method for the analysis of ochratoxin A in wine samples. Mobile phase composition consisted in methanol/aqueous component (0.1% phosphoric acid) in the ratio 60/40 (v/v). Temperature column was set tip to 20 degrees C. The sample clean-up and enrichment were achieved using the C-18 solid phase extraction (SPE) cartridges and compared to those achieved with immunoaffinity cartridges. The procedure based on C18 cartridges has been found to be cheaper, more robust and more convenient than the one based on immunoaffinity cartridges. The analytical method was characterized by a linearity domain situated within 0.1 - 10 mu g L-1 OTA in wine matrix, with a determination limit of 0.06 mu g L-1, for an injection volume of 50 mu L-1. The method was validated and applied to several Romanian wine samples of various proveniences.