Expression of active barley seed ribosome-inactivating protein in transgenic wheat

被引：61
|
作者：
Bieri, S [1 ]
Potrykus, I [1 ]
Fütterer, J [1 ]
机构：
[1] ETH Zurich, Inst Plant Sci, CH-8092 Zurich, Switzerland
来源：
THEORETICAL AND APPLIED GENETICS | 2000年 / 100卷 / 05期
关键词：
wheat transformation; ribosome-inactivating protein; anti-fungal protein; protein-targeting;
D O I：
10.1007/s001220051349
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Phenotypically normal, transgenic wheat (Triticum aestivum, var. Frisal) plants expressing a barley seed ribosome-inactivating protein (RIP) were produced. Expression was controlled by an intron-enhanced cauliflower mosaic virus 35S promoter and has been completely stable over four generations so far, possibly due to matrix-associated regions (MARs) that flank the transgenes. An engineered fusion to a signal peptide derived from the barley seed beta-1,3-glucanase caused the transport of RIP to the apoplast. Activity of the accumulated protein could be shown by significant inhibition of a rabbit reticulocyte transcription/translation system. Plants expressing high levels of RIP were protected only moderately or not at all against infection by the fungal pathogen Erysiphe graminis.
引用
收藏
页码：755 / 763
页数：9
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