A novel β-trefoil lectin from the parasol mushroom (Macrolepiota procera) is nematotoxic

被引:31
|
作者
Zurga, Simon [1 ]
Pohleven, Jure [1 ]
Renko, Miha [2 ,3 ]
Bleuler-Martinez, Silvia [4 ]
Sosnowski, Piotr [3 ]
Turk, Dusan [2 ,3 ]
Kuenzler, Markus [4 ]
Kos, Janko [1 ,5 ]
Sabotic, Jerica [1 ]
机构
[1] Jozef Stefan Inst, Dept Biotechnol, Jamova 39, SI-1000 Ljubljana, Slovenia
[2] Jozef Stefan Inst, Dept Biochem Mol & Struct Biol, SI-1000 Ljubljana, Slovenia
[3] Ctr Excellence Integrated Approaches Chem & Biol, Ljubljana, Slovenia
[4] Swiss Fed Inst Technol, Dept Biol, Inst Microbiol, Zurich, Switzerland
[5] Univ Ljubljana, Fac Pharm, Ljubljana 61000, Slovenia
基金
瑞士国家科学基金会;
关键词
basidiomycete; galactose; LacNAc; lectin; ricin B-like; CYSTEINE PROTEASE INHIBITORS; CLITOCYBE-NEBULARIS; STRUCTURAL-ANALYSIS; CRYSTAL-STRUCTURES; MARASMIUS-OREADES; FRUITING BODIES; HIGHER FUNGI; BINDING; COMPLEX; AGGLUTININ;
D O I
10.1111/febs.12875
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lectins are carbohydrate-binding proteins present in all organisms. Some cytoplasmic lectins from fruiting bodies of dikaryotic fungi are toxic against a variety of parasites and predators. We have isolated, cloned and expressed a novel, single domain lectin from Macrolepiota procera, designated MpL. Determination of the crystal structure revealed that MpL is a ricin B-like lectin with a beta-trefoil fold. Biochemical characterization, site-directed mutagenesis, co-crystallization with carbohydrates, isothermal titration calorimetry and glycan microarray analyses show that MpL forms dimers with the carbohydrate-binding site at the alpha-repeat, with the highest specificity for terminal N-acetyllactosamine and other beta-galactosides. A second putative carbohydrate-binding site with a low affinity for galactose is present at the gamma-repeat. In addition, a novel hydrophobic binding site was detected in MpL with specificity for molecules other than carbohydrates. The tissue specific distribution of MpL in the stipe and cap tissue of fruiting bodies and its toxicity towards the nematode Caenorhabditis elegans indicate a function of MpL in protecting fruiting bodies against predators and parasites.
引用
收藏
页码:3489 / 3506
页数:18
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