Antibody response to toxic shock syndrome toxin-1 of Staphylococcus aureus in dairy cows

Antibody response to toxic shock syndrome toxin-1 (TSST-1) of Staphylococcus aureus in dairy cows was examined by enzyme linked immunosorbent assay (ELISA). Serum antibody to TSST-1 was not detected in 39 (76.5%) of 51 calves, which were 1-6 months of age. In contrast, TSST-1 antibody was demonstrated in 1728 (72.6%) of 2380 lactating cows housed on 36 dairy farms. The ELISA values of antibody ranged from 0.2 to 3.0 OD and presented a distribution with the peak at 1.6 OD. The mean ELISA value differed between farms, and it increased slightly along with parturient history. Somatic cell counts of milk from 174 lactating cows was compared with TSST-1 antibody and tst gene examined by polymerase chain reaction (PCR). The mean ELISA values in sera were approximately the same value (1.143-1.297 OD) in cows, with somatic cell counts in milk ranging from <100,000 to >1,000,000 cells per mi. The mean ELISA values in milk were lower than those of sera, but they rose as somatic cells increased. The tst gene of S, aureus was detected in 76.0-86.2% of the milk samples containing somatic cells >500,000 cells per mi, a level which indicates mastitis. The data suggests that many lactating cows may be infected by TSST-1-producing S. aureus. (C) 2000 Elsevier Science B.V. All rights reserved.