Next Generation Indexing for Genomic Intervals

One-dimensional intervals incremental inverted index (Di4) is amulti-resolution, single-dimension indexing framework for efficient, scalable, and extensible computation of genomic interval expressions. The framework has a tri-layer architecture: the semantic layer provides orthogonal and genericmeans (including the support of user-defined function) of sense-making and higher-lever reasoning fromregion-based datasets; the logical layer provides building blocks for region calculus and topological relations between intervals; the physical layer abstracts from persistence technology and makes the model adaptable to variety of persistence technologies, spanning from small-scale (e.g., B+tree) to large-scale (e.g., LevelDB). The extensibility of Di4 to application scenarios is shown with an example of comparative evaluation of ChIP-seq and DNase-Seq replicates. Performance of Di4 is benchmarked for small and large scale scenarios under common bioinformatics application scenarios. Di4 is freely available from https://genometric.github.io/Di4.