Minor groove binding of a bis-quaternary ammonium compound: the crystal structure of SN 7167 bound to d(CGCGAATTCGCG)(2)

被引：18
|
作者：
Squire, CJ [1 ]
Clark, GR [1 ]
Denny, WA [1 ]
机构：
[1] UNIV AUCKLAND, SCH MED, CANC RES LAB, AUCKLAND, NEW ZEALAND
来源：
NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 20期
关键词：
D O I：
10.1093/nar/25.20.4072
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The X-ray crystal structure of the complex between the synthetic antitumour and antiviral DNA binding ligand SN 7167 and the DNA oligonucleotide d(CGCGAATTCGCG)(2) has been determined to an R factor of 18.3% at 2.6 Angstrom resolution, The ligand is located within the minor groove and covers almost 6 bp with the 1-methylpyridinium ring extending as far as the C9-G16 base pair and the 1-methylquinolinium ring lying between the G4-C21 and A5-T20 base pairs, The ligand interacts only weakly with the DNA, as evidenced by long range contacts and shallow penetration into the groove, This structure is compared with that of the complex between the parent compound SN 6999 and the alkylated DNA sequence d(CGC[e(6)G]AATTCGCG)(2). There are significant differences between the two structures in the extent of DNA bending, ligand conformation and groove binding.
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页码：4072 / 4078
页数：7
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